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Plant Physiol, February 2001, Vol. 125, pp. 1012-1022
Evidence That Intragenic Recombination Contributes to Allelic
Diversity of the S-RNase Gene at the Self-Incompatibility
(S) Locus in Petunia
inflata1
Xi
Wang,
Austin L.
Hughes,
Tatsuya
Tsukamoto,
Toshio
Ando, and
Teh-Hui
Kao*
Department of Biochemistry and Molecular Biology (X.W., T.-H.K.)
and Intercollege Graduate Degree Program in Plant Physiology (T.-H.K.),
Pennsylvania State University, University Park, Pennsylvania 16802;
Department of Biological Sciences, University of South Carolina,
Columbia, South Carolina 29208 (A.L.H.); and Faculty of
Horticulture, Chiba University, 648 Matsudo, Chiba 271-8510, Japan
(T.T., T.A.)
For Solanaceae type self-incompatibility, discrimination between
self and nonself pollen by the pistil is controlled by the highly
polymorphic S-RNase gene. To date, the mechanism generating the allelic
diversity of this gene is largely unknown. Natural populations offer a
good opportunity to address this question because they likely contain
different alleles that share recent common progenitors. We identified
19 S haplotypes from a natural population of
Petunia inflata in Argentina, used reverse
transcriptase-polymerase chain reaction to obtain cDNAs for 15 alleles
of the S-RNase gene, and sequenced all the cDNAs. Phylogenetic studies
revealed that five of these alleles and two previously identified
alleles form a major clade, and that the 5' region of
S19 allele was derived from an
ancestor allele closely related to S2, whereas its 3' region was derived from an ancestor allele closely related to S8. A similar evolutionary
relationship was found among S3,
S12, and
S15 alleles. These findings suggest
that intragenic recombination contributed to the generation of the
allelic diversity of the S-RNase gene. Two additional findings emerged
from the sequence comparisons. First, the nucleotide sequence of the
S1 allele identified in this work is
completely identical to that of the previously identified
S1 allele of a different origin.
Second, in the two hypervariable regions HVa and HVb, thought to be
involved in determining S allele specificity,
S6 and
S9 alleles differ only by four
nucleotides, all in HVb, resulting in two amino acid differences. The
implications of these findings are discussed.
1
This work was supported by the National Science
Foundation (grant nos. IBN-9603993 and IBN-9982659 to T.-H.K.) and by
the National Institutes of Health (grant no. GM34940 to
A.L.H.).
*
Corresponding author; e-mail: txk3{at}psu.edu; fax
814-863- 9416.
© 2001 American Society of Plant Physiologists
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