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Plant Physiol, February 2001, Vol. 125, pp. 1036-1044
Negative Interference of Endogenous Phytochrome B with
Phytochrome A Function in Arabidopsis1
Lars
Hennig,2
Christoph
Poppe,
Uta
Sweere,
Annette
Martin,3 and
Eberhard
Schäfer*
Institut für Biologie II, Universität Freiburg,
Schänzlestrasse 1, 79104 Freiburg, Germany
To study negative interactions between phytochromes, phytochrome B
(phyB) overexpressor lines, the mutants phyA-201,
phyB-4, phyB-5, phyD-1,
phyA-201 phyB-5, phyA-201 phyD-1, and
phyB-5 phyD-1 of Arabidopsis were used. Endogenous phyB,
but not phytochrome D (phyD), partly suppressed phytochrome A
(phyA)-dependent inhibition of hypocotyl elongation in far-red light
(FR). Dichromatic irradiation demonstrated that the negative effect of
phyB was largely independent of the photoequilibrium, i.e. far-red
light absorbing form of phytochrome formation. Moreover,
phyB-4, a mutant impaired in signal transduction, did
not show a loss of inhibition of phyA by phyB. Overexpression of phyB,
conversely, resulted in an enhanced inhibition of phyA function, even
in the absence of supplementary carbohydrates. However, overexpression
of a mutated phyB, which cannot incorporate the chromophore, had no
detectable effect on phyA action. In addition to seedling growth,
accumulation of anthocyanins in FR, another manifestation of the high
irradiance response, was strongly influenced by phyB holoprotein.
Induction of seed germination by FR, a very low fluence response, was
suppressed by both endogenous phyB and phyD. In conclusion, we show
that both classical response modes of phyA, high irradiance response, and very low fluence response are subject to an inhibitory action of
phyB-like phytochromes. Possible mechanisms of the negative interference are discussed.
1
This work was supported by the Deutsche Forschungsgemeinschaft.
2
Present address: Insitut für
Pflanzenwissenschaften, Eid genössische Technische
Hochschule Zürich, Universitätstrasse 2, 8092 Zurich, Switzerland.
3
Present address: Institut für Genetik, TU
München, Lichtenbergstrasse 4, 85747 Garching, Germany.
*
Corresponding author; e-mail schaegen{at}ruf.uni-freiburg.de; fax
49-761-203-2629.
© 2001 American Society of Plant Physiologists
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