|
Plant Physiol, February 2001, Vol. 125, pp. 1074-1085
Molecular Responses to Aphid Feeding in Arabidopsis in Relation
to Plant Defense Pathways1
Patrick J.
Moran and
Gary A.
Thompson2*
Center for Insect Science and Department of Plant Sciences,
University of Arizona, Tucson, Arizona 85721
Little is known about molecular responses in plants to phloem
feeding by insects. The induction of genes associated with wound and
pathogen response pathways was investigated following green peach aphid
(Myzus persicae) feeding on Arabidopsis. Aphid feeding on rosette leaves induced transcription of two genes associated with
salicylic acid (SA)-dependent responses to pathogens
(PR-1 and BGL2) 10- and 23-fold,
respectively. Induction of PR-1 and BGL2
mRNA was reduced in npr1 mutant plants, which are
deficient in SA signaling. Application of the SA analog
benzothiadiazole led to decreases in aphid reproduction on leaves of
both wild-type plants and mutant plants deficient in responsiveness to
SA, suggesting that wild-type SA-dependent responses do not influence
resistance to aphids. Two-fold increases occurred in mRNA levels of
PDF1.2, which encodes defensin, a peptide involved in
the jasmonate (JA)-/ethylene-dependent response pathway. Transcripts
encoding JA-inducible lipoxygenase (LOX2) and
SA/JA-inducible Phe-ammonia lyase increased 1.5- to 2-fold.
PDF1.2 and LOX2 induction by aphids did
not occur in infested leaves of the JA-resistant coi1-1
mutant. Aphid feeding induced 10-fold increases in mRNA levels of a
stress-related monosaccharide symporter gene, STP4.
Phloem feeding on Arabidopsis leads to stimulation of response pathways
associated with both pathogen infection and wounding.
1
This work was supported by a Research Training
Grant from the National Science Foundation (no. 9602249), by the
Southwest Consortium on Plant Genetics and Water Resources, and by the
University of Arizona Foundation.
2
Present address: Department of Applied Science,
University of Arkansas, 2801 South University Avenue, Little Rock, AR
72204-1099.
*
Corresponding author; email gathompson{at}ualr.edu; fax
501-569-8020.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
E. S. Villada, E. G. Gonzalez, A. I. Lopez-Sese, A. F. Castiel, and M. L. Gomez-Guillamon
Hypersensitive response to Aphis gossypii Glover in melon genotypes carrying the Vat gene
J. Exp. Bot.,
July 1, 2009;
60(11):
3269 - 3277.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Gosset, N. Harmel, C. Gobel, F. Francis, E. Haubruge, J.-P. Wathelet, P. du Jardin, I. Feussner, and M.-L. Fauconnier
Attacks by a piercing-sucking insect (Myzus persicae Sultzer) or a chewing insect (Leptinotarsa decemlineata Say) on potato plants (Solanum tuberosum L.) induce differential changes in volatile compound release and oxylipin synthesis
J. Exp. Bot.,
March 1, 2009;
60(4):
1231 - 1240.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. D. Girling, R. Madison, M. Hassall, G. M. Poppy, and J. G. Turner
Investigations into plant biochemical wound-response pathways involved in the production of aphid-induced plant volatiles
J. Exp. Bot.,
August 1, 2008;
59(11):
3077 - 3085.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. L. Walling
Avoiding Effective Defenses: Strategies Employed by Phloem-Feeding Insects
Plant Physiology,
March 1, 2008;
146(3):
859 - 866.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. B. Runyon, M. C. Mescher, and C. M. De Moraes
Parasitism by Cuscuta pentagona Attenuates Host Plant Defenses against Insect Herbivores
Plant Physiology,
March 1, 2008;
146(3):
987 - 995.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Abe, J. Ohnishi, M. Narusaka, S. Seo, Y. Narusaka, S. Tsuda, and M. Kobayashi
Function of Jasmonate in Response and Tolerance of Arabidopsis to Thrip Feeding
Plant Cell Physiol.,
January 1, 2008;
49(1):
68 - 80.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kusnierczyk, P. Winge, H. Midelfart, W. S. Armbruster, J. T. Rossiter, and A. M. Bones
Transcriptional responses of Arabidopsis thaliana ecotypes with different glucosinolate profiles after attack by polyphagous Myzus persicae and oligophagous Brevicoryne brassicae
J. Exp. Bot.,
July 11, 2007;
(2007)
erm043v2.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. I. Zarate, L. A. Kempema, and L. L. Walling
Silverleaf Whitefly Induces Salicylic Acid Defenses and Suppresses Effectual Jasmonic Acid Defenses
Plant Physiology,
February 1, 2007;
143(2):
866 - 875.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Kempema, X. Cui, F. M. Holzer, and L. L. Walling
Arabidopsis Transcriptome Changes in Response to Phloem-Feeding Silverleaf Whitefly Nymphs. Similarities and Distinctions in Responses to Aphids
Plant Physiology,
February 1, 2007;
143(2):
849 - 865.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kehr
Phloem sap proteins: their identities and potential roles in the interaction between plants and phloem-feeding insects
J. Exp. Bot.,
March 1, 2006;
57(4):
767 - 774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. A. Thompson and F. L. Goggin
Transcriptomics and functional genomics of plant defence induction by phloem-feeding insects
J. Exp. Bot.,
March 1, 2006;
57(4):
755 - 766.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Pegadaraju, C. Knepper, J. Reese, and J. Shah
Premature Leaf Senescence Modulated by the Arabidopsis PHYTOALEXIN DEFICIENT4 Gene Is Associated with Defense against the Phloem-Feeding Green Peach Aphid
Plant Physiology,
December 1, 2005;
139(4):
1927 - 1934.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Mewis, H. M. Appel, A. Hom, R. Raina, and J. C. Schultz
Major Signaling Pathways Modulate Arabidopsis Glucosinolate Accumulation and Response to Both Phloem-Feeding and Chewing Insects
Plant Physiology,
June 1, 2005;
138(2):
1149 - 1162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Klingler, R. Creasy, L. Gao, R. M. Nair, A. S. Calix, H. S. Jacob, O. R. Edwards, and K. B. Singh
Aphid Resistance in Medicago truncatula Involves Antixenosis and Phloem-Specific, Inducible Antibiosis, and Maps to a Single Locus Flanked by NBS-LRR Resistance Gene Analogs
Plant Physiology,
April 1, 2005;
137(4):
1445 - 1455.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-P. Dong, J. Peng, Z. Bao, X. Meng, J. M. Bonasera, G. Chen, S. V. Beer, and H. Dong
Downstream Divergence of the Ethylene Signaling Pathway for Harpin-Stimulated Arabidopsis Growth and Insect Defense
Plant Physiology,
November 1, 2004;
136(3):
3628 - 3638.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Zhu-Salzman, R. A. Salzman, J.-E. Ahn, and H. Koiwa
Transcriptional Regulation of Sorghum Defense Determinants against a Phloem-Feeding Aphid
Plant Physiology,
January 1, 2004;
134(1):
420 - 431.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. DEVOTO and J. G. TURNER
Regulation of Jasmonate-mediated Plant Responses in Arabidopsis
Ann. Bot.,
September 1, 2003;
92(3):
329 - 337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Hui, J. Iqbal, K. Lehmann, K. Gase, H. P. Saluz, and I. T. Baldwin
Molecular Interactions between the Specialist Herbivore Manduca sexta (Lepidoptera, Sphingidae) and Its Natural Host Nicotiana attenuata: V. Microarray Analysis and Further Characterization of Large-Scale Changes in Herbivore-Induced mRNAs
Plant Physiology,
April 1, 2003;
131(4):
1877 - 1893.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. D. Mikkelsen, B. L. Petersen, E. Glawischnig, A. B. Jensen, E. Andreasson, and B. A. Halkier
Modulation of CYP79 Genes and Glucosinolate Profiles in Arabidopsis by Defense Signaling Pathways
Plant Physiology,
January 1, 2003;
131(1):
298 - 308.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Li, M. M. Williams, Y.-T. Loh, G. I. Lee, and G. A. Howe
Resistance of Cultivated Tomato to Cell Content-Feeding Herbivores Is Regulated by the Octadecanoid-Signaling Pathway
Plant Physiology,
September 1, 2002;
130(1):
494 - 503.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. P. van Poecke and M. Dicke
Induced parasitoid attraction by Arabidopsis thaliana: involvement of the octadecanoid and the salicylic acid pathway
J. Exp. Bot.,
August 1, 2002;
53(375):
1793 - 1799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. J. Kliebenstein, A. Figuth, and T. Mitchell-Olds
Genetic Architecture of Plastic Methyl Jasmonate Responses in Arabidopsis thaliana
Genetics,
August 1, 2002;
161(4):
1685 - 1696.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. He, H. Fukushige, D. F. Hildebrand, and S. Gan
Evidence Supporting a Role of Jasmonic Acid in Arabidopsis Leaf Senescence
Plant Physiology,
March 1, 2002;
128(3):
876 - 884.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
