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Plant Physiol, February 2001, Vol. 125, pp. 1086-1093
Trehalose and Trehalase in Arabidopsis1
Joachim
Müller,23*
Roger A.
Aeschbacher,3
Astrid
Wingler,
Thomas
Boller, and
Andres
Wiemken
Botanisches Institut der Universität, Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland
Trehalase is ubiquitous in higher plants. So far, indications
concerning its function are scarce, although it has been implicated in
the detoxification of exogenous trehalose. A putative trehalase gene,
T19F6.15, has been identified in the genome sequencing
effort in Arabidopsis. Here we show that this gene encodes a functional trehalase when its cDNA is expressed in yeast, and that it is expressed
in various plant organs. Furthermore, we present results on the
distribution and activity of trehalase in Arabidopsis and we describe
how inhibition of trehalase by validamycin A affects the plants
response to exogenous trehalose ( -D-glucopyranosyl-[1, 1]--D-glucopyranoside). Trehalase activity was highest
in floral organs, particularly in the anthers (approximately 700 nkat g 1 protein) and maturing siliques (approximately 250 nkat g1 protein) and much lower in leaves, stems, and
roots (less than 50 nkat g1 protein). Inhibition of
trehalase in vivo by validamycin A led to the accumulation of an
endogenous substance that had all the properties of trehalose, and to a
strong reduction in sucrose and starch contents in flowers, leaves, and
stems. Thus, trehalose appears to be an endogenous substance in
Arabidopsis, and trehalose and trehalase may play a role in regulating
the carbohydrate allocation in plants.
1
This work was supported by the Swiss National
Science Foundation (grant nos. 3100-042535.94 to A.W. and
3100-040837.94 to T.B.) and by a fellowship from the Roche foundation
(to J.M.).
2
Present address: Friedrich Miescher Institute (FMI),
P.O. Box 2543, CH-4002 Basel, Switzerland.
3
These authors contributed equally to the paper.
*
Corresponding author; e-mail joachim.mueller{at}unibas.ch; fax
41-61-697-45-27.
© 2001 American Society of Plant Physiologists
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