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Plant Physiol, February 2001, Vol. 125, pp. 556-563
Brassinosteroid Levels Increase Drastically Prior to
Morphogenesis of Tracheary Elements
Ryo
Yamamoto,*
Shozo
Fujioka,
Taku
Demura,
Suguru
Takatsuto,
Shigeo
Yoshida, and
Hiroo
Fukuda
Department of Biological Sciences, Graduate School of Science, the
University of Tokyo, Tokyo 7-3-1, Japan (R.Y., T.D., H.F.); The RIKEN
(Institute of Physical and Chemical Research), Wako-shi, Saitama
351-0198, Japan (S.F., S.Y.); and Department of Chemistry, Joetsu
University of Education, Joetsu-shi, Niigata 943-8512, Japan
(S.T.)
As the first step toward understanding the involvement of
endogenous brassinosteroids (BRs) in cytodifferentiation, we analyzed biosynthetic activities of BRs in zinnia (Zinnia elegans
L. cv Canary Bird) cells differentiating into tracheary elements. The results of feeding experiments suggested that both the early and late
C6-oxidation pathways occur during tracheary element differentiation. Gas chromatography-mass spectrometry analysis revealed that five BRs,
castasterone, typhasterol, 6-deoxocastasterone, 6-deoxotyphasterol, and
6-deoxoteasterone, actually existed in cultured zinnia cells and
culture medium. Quantification of endogenous BRs in each stage of
tracheary element differentiation by gas chromatography-mass spectrometry exhibited that they increased dramatically prior to the
morphogenesis, which was consistent with the idea that BRs are
necessary for the initiation of the final stage of tracheary element
differentiation. Moreover, the proportion of each BR in culture medium
was quite different from that in cells, suggesting that specific BRs
are selectively secreted into medium and may function outside the cells.
*
Corresponding author; e-mail ss87221{at}mail.ecc.u-tokyo.ac.jp; fax
81-3-5841-4462.
© 2001 American Society of Plant Physiologists
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