|
Plant Physiol, February 2001, Vol. 125, pp. 564-572
Disruption of Microtubular Cytoskeleton Induced by Cryptogein, an
Elicitor of Hypersensitive Response in Tobacco
Cells1
Marie-Noëlle
Binet,*
Claude
Humbert,
David
Lecourieux,
Marylin
Vantard, and
Alain
Pugin
Unité Mixte de Recherche, Institut National de la Recherche
Agronomique, Université de Bourgogne, Biochimie, Biologie
Cellulaire et Ecologie des Interactions Plantes/Micro-Organismes, 17 Rue Sully, BV 86510, 21065 Dijon cedex, France (M.-N.B., C.H., D.L.,
A.P.); and Laboratoire de Physiologie Cellulaire Végétale,
Unité Mixte de Recherche, Commissariat à l'Energie
Atomique, Centre National de la Recherche Scientifique,
Université Joseph Fournier, 17 rue des Martyrs, 38054 Grenoble
cedex 9, France (M.V.)
The dynamics of microtubular cytoskeleton were studied in tobacco
(Nicotiana tabacum cv Xanthi) cells in response to two
different plant defense elicitors: cryptogein, a protein secreted by
Phytophthora cryptogea and oligogalacturonides (OGs),
derived from the plant cell wall. In tobacco plants cryptogein triggers
a hypersensitive-like response and induces systemic resistance against
a broad spectrum of pathogens, whereas OGs induce defense responses,
but fail to trigger cell death. The comparison of the microtubule (MT)
dynamics in response to cryptogein and OGs in tobacco cells indicates
that MTs appear unaffected in OG-treated cells, whereas cryptogein treatment caused a rapid and severe disruption of microtubular network.
When hyperstabilized by the MT depolymerization inhibitor, taxol, the
MT network was still disrupted by cryptogein treatment. On the other
hand, the MT-depolymerizing agent oryzalin and cryptogein had different
and complementary effects. In addition to MT destabilization, cryptogein induced the death of tobacco cells, whereas OG-treated cells
did not die. We demonstrated that MT destabilization and cell death
induced by cryptogein depend on calcium influx and that MT
destabilization occurs independently of active oxygen species
production. The molecular basis of cryptogein-induced MT disruption and
its potential significance with respect to cell death are discussed.
1
This work and D.L. were supported by the
Institut National de la Recherche Agronomique, by the Ministère
de l'Enseignement Supérieur et de la Recherche, and by the
Conseil Régional de Bourgogne.
*
Corresponding author; e-mail binet{at}dijon.inra.fr; fax
33-03-80-69-32-26.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
O. Krinke, M. Flemr, C. Vergnolle, S. Collin, J.-P. Renou, L. Taconnat, A. Yu, L. Burketova, O. Valentova, A. Zachowski, et al.
Phospholipase D Activation Is an Early Component of the Salicylic Acid Signaling Pathway in Arabidopsis Cell Suspensions
Plant Physiology,
May 1, 2009;
150(1):
424 - 436.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. S. Poulter, S. Vatovec, and V. E. Franklin-Tong
Microtubules Are a Target for Self-Incompatibility Signaling in Papaver Pollen
Plant Physiology,
March 1, 2008;
146(3):
1358 - 1367.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Higaki, T. Goh, T. Hayashi, N. Kutsuna, Y. Kadota, S. Hasezawa, T. Sano, and K. Kuchitsu
Elicitor-Induced Cytoskeletal Rearrangement Relates to Vacuolar Dynamics and Execution of Cell Death: In Vivo Imaging of Hypersensitive Cell Death in Tobacco BY-2 Cells
Plant Cell Physiol.,
October 1, 2007;
48(10):
1414 - 1425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ashtamker, V. Kiss, M. Sagi, O. Davydov, and R. Fluhr
Diverse Subcellular Locations of Cryptogein-Induced Reactive Oxygen Species Production in Tobacco Bright Yellow-2 Cells
Plant Physiology,
April 1, 2007;
143(4):
1817 - 1826.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yoda, Y. Hiroi, and H. Sano
Polyamine Oxidase Is One of the Key Elements for Oxidative Burst to Induce Programmed Cell Death in Tobacco Cultured Cells
Plant Physiology,
September 1, 2006;
142(1):
193 - 206.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Lamotte, K. Gould, D. Lecourieux, A. Sequeira-Legrand, A. Lebrun-Garcia, J. Durner, A. Pugin, and D. Wendehenne
Analysis of Nitric Oxide Signaling Functions in Tobacco Cells Challenged by the Elicitor Cryptogein
Plant Physiology,
May 1, 2004;
135(1):
516 - 529.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kadota, T. Goh, H. Tomatsu, R. Tamauchi, K. Higashi, S. Muto, and K. Kuchitsu
Cryptogein-Induced Initial Events in Tobacco BY-2 Cells: Pharmacological Characterization of Molecular Relationship among Cytosolic Ca2+ Transients, Anion Efflux and Production of Reactive Oxygen Species
Plant Cell Physiol.,
February 15, 2004;
45(2):
160 - 170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Olivain, S. Trouvelot, M.-N. Binet, C. Cordier, A. Pugin, and C. Alabouvette
Colonization of Flax Roots and Early Physiological Responses of Flax Cells Inoculated with Pathogenic and Nonpathogenic Strains of Fusarium oxysporum
Appl. Envir. Microbiol.,
September 1, 2003;
69(9):
5453 - 5462.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Baillieul, P. de Ruffray, and S. Kauffmann
Molecular Cloning and Biological Activity of alpha -, beta -, and gamma -Megaspermin, Three Elicitins Secreted by Phytophthora megasperma H20
Plant Physiology,
January 1, 2003;
131(1):
155 - 166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Bourque, R. Lemoine, A. Sequeira-Legrand, L. Fayolle, S. Delrot, and A. Pugin
The Elicitor Cryptogein Blocks Glucose Transport in Tobacco Cells
Plant Physiology,
December 1, 2002;
130(4):
2177 - 2187.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Lecourieux, C. Mazars, N. Pauly, R. Ranjeva, and A. Pugin
Analysis and Effects of Cytosolic Free Calcium Increases in Response to Elicitors in Nicotiana plumbaginifolia Cells
PLANT CELL,
October 1, 2002;
14(10):
2627 - 2641.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Wendehenne, O. Lamotte, J.-M. Frachisse, H. Barbier-Brygoo, and A. Pugin
Nitrate Efflux Is an Essential Component of the Cryptogein Signaling Pathway Leading to Defense Responses and Hypersensitive Cell Death in Tobacco
PLANT CELL,
August 1, 2002;
14(8):
1937 - 1951.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
