Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (36)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Huang, S.
Right arrow Articles by Brown, S. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, S.
Right arrow Articles by Brown, S. M.
Agricola
Right arrow Articles by Huang, S.
Right arrow Articles by Brown, S. M.

Plant Physiol, February 2001, Vol. 125, pp. 573-584

Cloning of an Arabidopsis Patatin-Like Gene, STURDY, by Activation T-DNA Tagging

Shihshieh Huang,* R. Eric Cerny, Deepti S. Bhat, and Sherri M. Brown

Mystic Research, Monsanto Company, 62 Maritime Drive, Mystic, Connecticut 06355 (S.H.); and Agricultural Sector, Monsanto Company, 700 Chesterfield Parkway North, St. Louis, Missouri 63198 (R.E.C., D.S.B., S.M.B.)

Activation T-DNA tagging can generate dominant gain-of-function mutants by overexpression of a particular endogenous gene. We identified an activation-tagged mutant, sturdy, exhibiting a stiff inflorescence stem, thicker leaves, shorter siliques, larger seeds, round-shaped flowers, and delayed growth. It is most important that unlike its wild-type counterpart, this mutant is less prone to lodging. Cloning of STURDY revealed that in sturdy, there is an open reading frame containing a single intron encoding a patatin-like homolog. The T-DNA is inserted into the 3' region of the second exon. The mutant phenotype was shown to be the result of overexpression of STURDY by mRNA analysis and transgenic studies. Preliminary histological studies have revealed an increase in cell number in the inflorescence stem of mutant plants; however, additional studies are needed to better understand the overexpression phenotype.

* Corresponding author; e-mail shihshieh.huang{at}monsanto.com; fax 860-572-5240.

© 2001 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 J Exp BotHome page
K. Petroni, G. Falasca, V. Calvenzani, D. Allegra, C. Stolfi, L. Fabrizi, M. M. Altamura, and C. Tonelli
The AtMYB11 gene from Arabidopsis is expressed in meristematic cells and modulates growth in planta and organogenesis in vitro
J. Exp. Bot., April 1, 2008; 59(6): 1201 - 1213.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Yu, X. Chen, Y.-Y. Hong, Y. Wang, P. Xu, S.-D. Ke, H.-Y. Liu, J.-K. Zhu, D. J. Oliver, and C.-B. Xiang
Activated Expression of an Arabidopsis HD-START Protein Confers Drought Tolerance with Improved Root System and Reduced Stomatal Density
PLANT CELL, April 1, 2008; 20(4): 1134 - 1151.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Ezaki, H. Kiyohara, H. Matsumoto, and S. Nakashima
Overexpression of an auxilin-like gene (F9E10.5) can suppress Al uptake in roots of Arabidopsis
J. Exp. Bot., February 1, 2007; 58(3): 497 - 506.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Y. Kim, K. Tillison, J.-H. Lee, D. A. Rearick, and C. M. Smas
The adipose tissue triglyceride lipase ATGL/PNPLA2 is downregulated by insulin and TNF-{alpha} in 3T3-L1 adipocytes and is a target for transactivation by PPAR{gamma}
Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E115 - E127.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
H. Mathews, S. K. Clendennen, C. G. Caldwell, X. L. Liu, K. Connors, N. Matheis, D. K. Schuster, D. J. Menasco, W. Wagoner, J. Lightner, et al.
Activation Tagging in Tomato Identifies a Transcriptional Regulator of Anthocyanin Biosynthesis, Modification, and Transport
PLANT CELL, August 1, 2003; 15(8): 1689 - 1703.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
P. R. SHEWRY
Tuber Storage Proteins
Ann. Bot., June 1, 2003; 91(7): 755 - 769.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
K.-H. Jung, J. Hur, C.-H. Ryu, Y. Choi, Y.-Y. Chung, A. Miyao, H. Hirochika, and G. An
Characterization of a Rice Chlorophyll-Deficient Mutant Using the T-DNA Gene-Trap System
Plant Cell Physiol., May 15, 2003; 44(5): 463 - 472.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D.-H. Jeong, S. An, H.-G. Kang, S. Moon, J.-J. Han, S. Park, H. S. Lee, K. An, and G. An
T-DNA Insertional Mutagenesis for Activation Tagging in Rice
Plant Physiology, December 1, 2002; 130(4): 1636 - 1644.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Holk, S. Rietz, M. Zahn, H. Quader, and G. F.E. Scherer
Molecular Identification of Cytosolic, Patatin-Related Phospholipases A from Arabidopsis with Potential Functions in Plant Signal Transduction
Plant Physiology, September 1, 2002; 130(1): 90 - 101.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Marsch-Martinez, R. Greco, G. Van Arkel, L. Herrera-Estrella, and A. Pereira
Activation Tagging Using the En-I Maize Transposon System in Arabidopsis
Plant Physiology, August 1, 2002; 129(4): 1544 - 1556.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2001 by the American Society of Plant Biologists