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Plant Physiol, February 2001, Vol. 125, pp. 627-633
Gibberellin Biosynthesis Mutations and Root Development in
Pea
Julian R.
Yaxley,
John J.
Ross,*
Leanne J.
Sherriff, and
James B.
Reid
School of Plant Science, G.P.O. Box 252-55, University of
Tasmania, Hobart, Tasmania 7001, Australia
Dwarf mutants of pea (Pisum sativum), with impaired
gibberellin (GA) biosynthesis in the shoot, were studied to determine whether the roots of these genotypes had altered elongation and GA
levels. Mutations na, lh-2, and
ls-1 reduced GA levels in root tips and taproot
elongation, although in lh-2 and ls-1
roots the reduction in elongation was small (less than 15%). The
na mutation reduced taproot length by about 50%. The
roots of na plants elongated in response to applied
GA1 and recombining na with mutation
sln (which blocks GA catabolism) increased
GA1 levels in root tips and completely restored normal root
development. In shoots, Mendel's le-1 mutation impairs
the 3 -hydroxylation of GA20 to the bioactive GA1, resulting in dwarfism. However, GA1 and
GA20 levels were normal in le-1 roots, as
was root development. The null mutation le-2 also did
not reduce root GA levels or elongation. The results support the theory
that GAs are important for normal root elongation in pea, and indicate
that a 3-hydroxylase gene other than LE operates in
pea roots.
*
Corresponding author; e-mail John.Ross{at}utas.edu.au; fax
61-3-62-262698.
© 2001 American Society of Plant Physiologists
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