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Plant Physiol, February 2001, Vol. 125, pp. 701-710
Molecular Interactions between the Specialist Herbivore
Manduca sexta (Lepidoptera, Sphingidae) and Its Natural
Host Nicotiana attenuata. II. Accumulation of Plant
mRNAs in Response to Insect-Derived Cues1
Ursula
Schittko,
Dieter
Hermsmeier,2 and
Ian T.
Baldwin*
Department of Molecular Ecology, Max Planck Institute for Chemical
Ecology, Carl Zeiss Promenade 10, D-07745 Jena, Germany
The transcriptional changes in Nicotiana attenuata
Torr. ex Wats. elicited by attack from Manduca sexta
larvae were previously characterized by mRNA differential display (D. Hermsmeier, U. Schittko, I.T. Baldwin [2001] Plant Physiol 125:
683-700). Because herbivore attack causes wounding, we
disentangled wound-induced changes from those elicited by M.
sexta oral secretions and regurgitant (R) with a northern
analysis of a subset of the differentially expressed transcripts
encoding threonine deaminase, pathogen-induced oxygenase, a photosystem
II light-harvesting protein, a retrotransposon homolog, and three
unknown genes. R extensively modified wound-induced responses by
suppressing wound-induced transcripts (type I) or amplifying the
wound-induced response (type II) further down-regulating wound-suppressed transcripts (type IIa) or up-regulating wound-induced transcripts (type IIb). It is interesting that although all seven genes
displayed their R-specific patterns in the treated tissues largely
independently of the leaf or plant developmental stage, only the type I
genes displayed strong systemic induction. Ethylene was not responsible
for any of the specific patterns of expression. R collected from
different tobacco feeding insects, M. sexta, Manduca quinquemaculata, and Heliothis
virescens, as well as from different instars of M.
sexta were equally active. The active components of M.
sexta R were heat stable and active in minute amounts,
comparable with real transfer rates during larval feeding. Specific
expression patterns may indicate that the plant is adjusting its wound
response to efficiently fend off M. sexta, but may also be advantageous to the larvae, especially when R suppress wound-induced plant responses.
1
This work was supported by the Max Planck Gesellschaft.
2
Present address: Department of Genetics and
Evolution, Max Planck Institute for Chemical Ecology, Carl Zeiss
Promenade 10, D-07745 Jena, Germany
*
Corresponding author; e-mail Baldwin{at}ice.mpg.de; fax
49-3641-643653.
© 2001 American Society of Plant Physiologists
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