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Plant Physiol, February 2001, Vol. 125, pp. 728-737
LEPS2, a Phosphorus
Starvation-Induced Novel Acid Phosphatase from
Tomato1
James C.
Baldwin,
Athikkattuvalasu S.
Karthikeyan, and
Kashchandra G.
Raghothama*
Department of Horticulture and Landscape Architecture, Purdue
University, West Lafayette, Indiana 47907-1165
Phosphate (Pi) is one of the least available plant nutrients
found in the soil. A significant amount of phosphate is bound in
organic forms in the rhizosphere. Phosphatases produced by plants and
microbes are presumed to convert organic phosphorus into available Pi,
which is absorbed by plants. In this study we describe the isolation
and characterization of a novel tomato (Lycopersicon
esculentum) phosphate starvation-induced gene
(LePS2) representing an acid phosphatase.
LePS2 is a member of a small gene family in tomato. The
cDNA is 942 bp long and contains an open reading frame encoding a
269-amino acid polypeptide. The amino acid sequence of LePS2 has a
significant similarity with a phosphatase from chicken. Distinct
regions of the peptide also share significant identity with the members
of HAD and DDDD super families of phosphohydrolases. Many plant
homologs of LePS2 are found in the databases. The
LePS2 transcripts are induced rapidly in tomato plant
and cell culture in the absence of Pi. However, the induction is
repressible in the presence of Pi. Divided root studies indicate that
internal Pi levels regulate the expression of LePS2. The
enhanced expression of LePS2 is a specific response to
Pi starvation, and it is not affected by starvation of other nutrients
or abiotic stresses. The bacterially (Escherichia coli) expressed protein exhibits phosphatase activity against the synthetic substrate p-nitrophenyl phosphate. The pH optimum of the
enzyme activity suggests that LePS2 is an acid phosphatase.
1
This research is funded in part by the U.S.
Department of Agriculture-National Research Initiative Competitive
Grants Program (grant no. 97-35100-4211). This is journal paper no.
16,382 of the Purdue University Agricultural Research Programs.
*
Corresponding author; e-mail ragu{at}hort.purdue.edu; fax
765-494-0391.
© 2001 American Society of Plant Physiologists
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