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Plant Physiol, February 2001, Vol. 125, pp. 787-796
The Calcium Rhythms of Different Cell Types Oscillate with
Different Circadian Phases
Nicola T.
Wood,23*
Ann
Haley,2
Martine
Viry-Moussaïd,
Carl H.
Johnson,
Arnold H.
van der
Luit,4 and
Anthony J.
Trewavas
Institute of Cell and Molecular Biology, Kings Buildings, Mayfield
Road, University of Edinburgh, Edinburgh EH9 3JH, United Kingdom
(N.T.W., A.H., M.V.-M., A.H.v.d.L., A.J.T.); and Department of Biology,
Box 1812-B, Vanderbilt University, Nashville, Tennessee 37235 (C.H.J.)
Transgenic tobacco (Nicotiana plumbaginifolia)
seedlings containing the Ca2+-sensitive luminescent protein
aequorin have been shown to exhibit circadian variations in cytosolic
calcium. Concomitant measurements of cytosolic and nuclear calcium show
that circadian variations in the cytoplasm are not expressed in the
nucleus. To investigate whether all cells of transgenic seedlings
contribute equally to circadian variations in cytosolic calcium,
different promoters eliciting different expression patterns have been
placed upstream of aequorin and used for transformation. The circadian
peak occurred at different times in the three transgenic lines
constructed. Luminescence imaging of these transgenic lines indicated
that aequorin was differentially accumulated among the main tissues and
cells of the seedlings and overcoat technology with applied epidermal
strips indicated that the surface cell layers contribute the vast
majority of luminescent light. We conclude that the Ca2+
rhythmicities of cells and tissues oscillate with distinct differences in phase, that this might represent different underlying cellular control mechanisms and that these observations have significant implications for our understanding and study of Ca2+
mediated signal transduction in plant cells.
2
These authors contributed equally to the paper.
3
Present address: Division of Pathology, Unit of
Virology, Scottish Crop Research Institute, Invergowrie, Dundee DD2
5DA, UK.
4
Present address: Institute for Molecular Cell Biology,
University of Amsterdam, Kruislaan 318, 1098 SM Amsterdam, The Netherlands.
*
Corresponding author; e-mail nwood{at}scri.sari.ac.uk; fax
44-01382-562426.
© 2001 American Society of Plant Physiologists
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