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Plant Physiol, February 2001, Vol. 125, pp. 877-890
Sugar Coordinately and Differentially Regulates Growth- and
Stress-Related Gene Expression via a Complex Signal Transduction
Network and Multiple Control Mechanisms1
Shin-Lon
Ho,
Yu-Chan
Chao,
Wu-Fu
Tong, and
Su-May
Yu*
Institute of Molecular Biology, Academia Sinica, Nankang, Taipei,
Taiwan 11529, Republic of China (S.-L.H., Y.-C.C., S.-M.Y.); and
Department of Biology, National Taiwan Normal University, Taipei,
Taiwan 10764, Republic of China (S.-L.H., W.-F.T.)
In plants, sugars are required to sustain growth and regulate gene
expression. A large set of genes are either up- or down-regulated by
sugars; however, whether there is a common mechanism and signal transduction pathway for differential and coordinated sugar regulation remain unclear. In the present study, the rice (Oryza
sativa cv Tainan 5) cell culture was used as a model system to
address this question. Sucrose and glucose both played dual functions
in gene regulation as exemplified by the up-regulation of
growth-related genes and down-regulation of stress-related genes. Sugar
coordinately but differentially activated or repressed gene expression,
and nuclear run-on transcription and mRNA half-life analyses revealed regulation of both the transcription rate and mRNA stability. Although
coordinately regulated by sugars, these growth- and stress-related genes were up-regulated or down-regulated through hexokinase-dependent and/or hexokinase-independent pathways. We also found that the sugar
signal transduction pathway may overlap the glycolytic pathway for gene
repression.  -Amylase and the stress-related genes identified in this
study were coordinately expressed under sugar starvation, suggesting a
convergence of the nutritional and environmental stress signal
transduction pathways. Together, our studies provide a new insight into
the complex signal transduction network and mechanisms of sugar
regulation of growth and stress-related genes in plants.
1
This work was supported by the Academia Sinica,
the National Science Council (grant no. NSC 89-2311-B-001-023), and
the Biomedical Research Foundation of the Republic of China.
*
Corresponding author; e-mail sumay{at}ccvax.sinica.edu.tw; fax
886-2-2788-2695 or 886-2-2782-6085.
© 2001 American Society of Plant Physiologists
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