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Plant Physiol, February 2001, Vol. 125, pp. 982-989
Photosynthetic Capacity Is Differentially Affected by Reductions
in Sedoheptulose-1,7-Bisphosphatase Activity during Leaf Development
in Transgenic Tobacco Plants1
Hülya
Ölçer,2
Julie C.
Lloyd, and
Christine A.
Raines*
Department of Biological Sciences, University of Essex, Colchester
CO4 3SQ, United Kingdom
The impact of reduced sedoheptulose-1,7-bisphosphatase (SBPase)
activity on photosynthetic capacity and carbohydrate status was
examined during leaf expansion and maturation in antisense transgenic
tobacco (Nicotiana tabacum L. cv Samsun) plants. In wild-type plants, photosynthetic capacity was lowest in young expanding
leaves and reached a maximum in the fully expanded, mature leaves. In
contrast, the transgenic antisense SBPase plants had the highest
photosynthetic rates in the young expanding leaves and lowest rates in
the mature leaves. In the mature, fully expanded leaves of the
transgenic plants photosynthetic capacity was closely correlated with
the level of SBPase activity. However, in the youngest leaves of the
SBPase antisense plants, photosynthetic rates were close to, or higher
than, those observed in wild-type plants, despite having a lower SBPase
activity than the equivalent wild-type leaves. Reductions in SBPase
activity affected carbohydrate levels in both the mature and young
developing leaves. The overall trend was for decreased SBPase activity
to lead to reductions in carbohydrate levels, particularly in starch.
However, these changes in carbohydrate content were also dependent on
the developmental status of the leaf. For example, in young expanding
leaves of plants with the smallest reductions in SBPase activity, the
levels of starch were higher than in wild-type plants. These data
suggest that the source status of the mature leaves is an important
determinant of photosynthetic development.
1
This work was supported by funding from the
Biotechnology and Biological Sciences Research Council, United Kingdom
(grant no. P01723 to J.C.L. and C.A.R.). H.O. was supported by funding from T.C. Dumlupinar University, which was provided by the
Turkish Higher Educational Counselor (YÖK).
2
Present address: Dumlupinar Üniversitesi, Fen
Edebiyat Fakültesi, Biyoloji Bolümü, Kütahya, Turkey.
*
Corresponding author; e-mail rainc{at}essex.ac.uk; fax
44-1206- 872592.
© 2001 American Society of Plant Physiologists
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