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Plant Physiol, March 2001, Vol. 125, pp. 1216-1227
A Complete Set of Maize Individual Chromosome Additions to the
Oat Genome1
Ralf G.
Kynast,
Oscar
Riera-Lizarazu,2
M. Isabel
Vales,2
Ron J.
Okagaki,
Silvia B.
Maquieira,
Gang
Chen,
Evgueni V.
Ananiev,3
Wade E.
Odland,
Charles D.
Russell,
Adrian O.
Stec,
Suzanne M.
Livingston,
Herika A.
Zaia,
Howard W.
Rines, and
Ronald L.
Phillips*
Plant Molecular Genetics Institute (R.G.K., O.R.-L., M.I.V.,
R.J.O., S.B.M., G.C., E.V.A., W.E.O., C.D.R., A.O.S., S.M.L., H.A.Z.,
R.L.P.) and Plant Science Research Unit, United States Department of
Agriculture-Agricultural Research Service (USDA-ARS) (H.W.R.),
Department of Agronomy and Plant Genetics, University of Minnesota, 411 Borlaug Hall, 1991 Buford Circle, St. Paul, Minnesota 55108-6026
All 10 chromosomes of maize (Zea mays,
2n = 2x = 20) were recovered as single
additions to the haploid complement of oat (Avena sativa, 2n = 6x = 42) among
F1 plants generated from crosses involving three different
lines of maize to eight different lines of oat. In vitro rescue culture
of more than 4,300 immature F1 embryos resulted in a
germination frequency of 11% with recovery of 379 F1
plantlets (8.7%) of moderately vigorous growth. Some F1
plants were sectored with distinct chromosome constitutions among
tillers of the same plant and also between root and shoot cells.
Meiotic restitution facilitated development of un-reduced gametes in
the F1. Self-pollination of these partially fertile
F1 plants resulted in disomic additions
(2n = 6x + 2 = 44) for maize chromosomes 1, 2, 3, 4, 6, 7, and 9. Maize chromosome 8 was recovered as a monosomic
addition (2n = 6x + 1 = 43). Monosomic
additions for maize chromosomes 5 and 10 to a haploid complement of oat
(n = 3x + 1 = 22) were recovered several times
among the F1 plants. Although partially fertile, these
chromosome 5 and 10 addition plants have not yet transmitted the added
maize chromosome to F2 offspring. We discuss the
development and general utility of this set of oat-maize addition lines
as a novel tool for maize genomics and genetics.
1
This work was supported by the National Science
Foundation (grant no. 9872650) and is a joint contribution of the
Minnesota Agricultural Experiment Station and USDA-ARS. Mention of
trademark or proprietary product does not constitute a guarantee or
warranty by the University of Minnesota or USDA-ARS and does not imply approval over other products that also may be suitable.
2
Present address: Department of Crop and Soil Sciences,
Oregon State University, 107 Crop Science Building, Corvallis, OR
97331-3002.
3
Present address: Trait and Technology Development,
Pioneer Hi-Bred International, Inc., 7300 NW 62nd Avenue, P.O. Box
1004, Johnston, IA 50131-1004.
*
Corresponding author; e-mail phill005{at}umn.edu; fax
612-625-1268.
© 2001 American Society of Plant Physiologists
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