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Plant Physiol, March 2001, Vol. 125, pp. 1248-1257
Screening of the Rice Viviparous Mutants Generated by Endogenous
Retrotransposon Tos17 Insertion. Tagging of a
Zeaxanthin Epoxidase Gene and a Novel OsTATC
Gene1
Ganesh Kumar
Agrawal,
Muneo
Yamazaki,
Masatomo
Kobayashi,
Rei
Hirochika,
Akio
Miyao, and
Hirohiko
Hirochika*
Department of Molecular Genetics, National Institute of
Agrobiological Resources, Tsukuba, Ibaraki 305-8602, Japan (G.K.A.,
M.Y., R.H., A.M., H.H.); and Laboratory of Plant Molecular Biology,
RIKEN Tsukuba Institute, Tsukuba, Ibaraki 305-0074, Japan
(M.K.)
The rice (Oryza sativa) retrotransposon
Tos17 is one of a few active retrotransposons in plants
and its transposition is activated by tissue culture. Here, we present
the characterization of viviparous mutants of rice induced by tissue
culture to demonstrate the feasibility of the use of retrotransposon
Tos17 as an endogenous insertional mutagen and cloning
of the tagged gene for forward genetics in unraveling the gene
function. Two mutants were shown to be caused by the insertion of
Tos17. Osaba1, a strong viviparous mutant with wilty
phenotype, displayed low abscisic acid level and almost no further
increase in its levels upon drought. The mutant is shown to be impaired
in the epoxidation of zeaxanthin. On the other hand,
Ostatc, a mutant with weak phenotype, exhibited the pale
green phenotype and slight increase in abscisic acid levels upon
drought. Deduced amino acids of the causative genes of
Osaba1 and Ostatc manifested a
significantly high homology with zeaxanthin epoxidase isolated from
other plant species and with bacterial Sec-independent translocase TATC
protein, respectively. This is the first example of transposon tagging
in rice.
1
This work was supported by the Ministry of
Agriculture, Forestry, and Fisheries of Japan; by a grant for the
enhancement of Center-of-Excellence; by the special coordination funds
for promoting Science and Technology in Japan; and by the Program for
Promotion of Basic Research Activities for Innovative Biosciences (to
G.K.A.).
*
Corresponding author; e-mail hirohiko{at}abr.affrc.go.jp; fax
81-298-38-7408.
© 2001 American Society of Plant Physiologists
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