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Plant Physiol, March 2001, Vol. 125, pp. 1271-1282

Quantitative Trait Locus Mapping of Loci Influencing Elongation Factor 1alpha Content in Maize Endosperm1

Xuelu Wang, Young-min Woo, Cheol Soo Kim, and Brian A. Larkins*

Department of Plant Sciences, University of Arizona, Tucson, Arizona 85721

The nutritional value of maize (Zea mays) seed is most limited by its protein quality because its storage proteins are devoid of the essential amino acid lysine (Lys). The Lys content of the kernel can be significantly increased by the opaque-2 mutation, which reduces zein synthesis and increases accumulation of proteins that contain Lys. Elongation factor 1alpha (eEF1A) is one of these proteins, and its concentration is highly correlated with the Lys content of the endosperm. We investigated the genetic regulation of eEF1A and the basis for its relationship with other Lys-containing proteins by analyzing the progeny of a cross between a high (Oh51Ao2) and a low (Oh545o2) eEF1A maize inbred. We identified 83 simple sequence repeat loci that are polymorphic between these inbreds; the markers are broadly distributed over the genome (1,402 cM) with an average interval of 17 cM. Genotypic analysis of the F2 progeny revealed two significant quantitative trait loci that account for 25% of the variance for eEF1A content. One of these is on the short arm of chromosome 4 and is linked with a cluster of 22-kD alpha -zein coding sequences; the other quantitative trait locus is on the long arm of chromosome 7. The content of alpha -zein and gamma -zein was measured in pools of high- and low-eEF1A individuals obtained from this cross, and a higher level of alpha -zein was found to cosegregate with high eEF1A content. Allelic variation at the 22-kD alpha -zein locus may contribute to the difference of eEF1A content between Oh51Ao2 and Oh545o2 by increasing the surface area of protein bodies in the endosperm and creating a more extensive network of cytoskeletal proteins.

1 This work was supported by the U.S. Department of Agriculture National Research Initiative (grant no. NRI 981427 to B.A.L.).

* Corresponding author; e-mail Larkins{at}Ag.Arizona.edu; fax 520-621-3692.

© 2001 American Society of Plant Physiologists


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