|
Plant Physiol, March 2001, Vol. 125, pp. 1380-1387
Role of mRNA Secondary Structure in Translational Repression of
the Maize Transcriptional Activator
Lc1,2
Liangjiang
Wang and
Susan R.
Wessler*
Departments of Botany and Genetics, University of Georgia, Athens,
Georgia 30602
Lc, a member of the maize (Zea mays)
R/B gene family, encodes a basic helix-loop-helix
transcriptional activator of the anthocyanin biosynthetic pathway. It
was previously shown that translation of the Lc mRNA is
repressed by a 38-codon upstream open reading frame (uORF) in the 5'
leader. In this study, we report that a potential hairpin structure
near the 5'end of the Lc mRNA also represses downstream
translation in the rabbit reticulocyte in vitro translation system and
in transient transformation assays. Base pairing of the hairpin is
important for repression because its destabilization increases
translation of the uORF and the downstream ORF. However, translation of
the uORF is not required for the hairpin-mediated repression. Instead,
the uORF and the 5'-proximal hairpin mediate two independent levels of
repression. Although the uORF represses downstream translation due to
inefficient reinitiation of ribosomes that translate uORF, the hairpin
inhibits ribosome loading at the 5' end of the mRNA.
1
This work was supported by the U.S. Department
of Energy (grant to S.R.W.).
2
We dedicate this paper to the memory of Dr. Alan Jaworski.
*
Corresponding author; e-mail sue{at}dogwood.botany.uga.edu; fax
706-542-1805.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
L. Koreen, S. V. Ramaswamy, S. Naidich, I. V. Koreen, G. R. Graff, E. A. Graviss, and B. N. Kreiswirth
Comparative Sequencing of the Serine-Aspartate Repeat-Encoding Region of the Clumping Factor B Gene (clfB) for Resolution within Clonal Groups of Staphylococcus aureus
J. Clin. Microbiol.,
August 1, 2005;
43(8):
3985 - 3994.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. David-Assael, H. Saul, V. Saul, T. Mizrachy-Dagri, I. Berezin, E. Brook, and O. Shaul
Expression of AtMHX, an Arabidopsis vacuolar metal transporter, is repressed by the 5' untranslated region of its gene
J. Exp. Bot.,
March 1, 2005;
56(413):
1039 - 1047.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Ray, M. Yu, P. Auser, L. Blahut-Beatty, B. McKersie, S. Bowley, N. Westcott, B. Coulman, A. Lloyd, and M. Y. Gruber
Expression of Anthocyanins and Proanthocyanidins after Transformation of Alfalfa with Maize Lc
Plant Physiology,
July 1, 2003;
132(3):
1448 - 1463.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. S. Watson, V. S. Asirvatham, L. Wang, and L. W. Sumner
Mapping the Proteome of Barrel Medic (Medicago truncatula)
Plant Physiology,
March 1, 2003;
131(3):
1104 - 1123.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Hanfrey, M. Franceschetti, M. J. Mayer, C. Illingworth, and A. J. Michael
Abrogation of Upstream Open Reading Frame-mediated Translational Control of a Plant S-Adenosylmethionine Decarboxylase Results in Polyamine Disruption and Growth Perturbations
J. Biol. Chem.,
November 8, 2002;
277(46):
44131 - 44139.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. B. Lai, S. L. Tausta, and T. M. Nelson
Differential Regulation of Transcripts Encoding Cytosolic NADP-Malic Enzyme in C3 and C4Flaveria Species
Plant Physiology,
January 1, 2002;
128(1):
140 - 149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
