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Plant Physiol, March 2001, Vol. 125, pp. 1388-1395
Nonsense-Mediated Decay of Mutant waxy mRNA in
Rice
Masayuki
Isshiki,
Yoshiaki
Yamamoto,
Hikaru
Satoh, and
Ko
Shimamoto*
Laboratory of Plant Molecular Genetics, Nara Institute of Science
and Technology, 8916-5 Takayama, Ikoma 630-0101, Japan
(M.I., Y.Y., K.S.); and Faculty of Agriculture, Kyushu University,
Hakozaki, Fukuoka 812, Japan (H.S.)
Two rice (Oryza sativa) waxy mutations of
the Japonica background were shown to contain approximately 20% of the
fully spliced mRNA relative to the wild type. Sequencing analysis of
the entire waxy genes of the two mutants revealed the
presence of premature translation termination codons in exon 2 and exon
7. These results indicated that the lower accumulation of fully spliced
RNA in the mutants was caused by nonsense-mediated decay (NMD), which is an RNA surveillance system universally found in eukaryotes. It is
interesting that levels of RNA retaining intron 1 were not changed by
premature nonsense codons, suggesting that splicing may be linked with
NMD in plants, as previously found in mammalian cells. Measurements of
the half-lives of waxy RNAs in transfected rice
protoplasts indicated that the half-life of waxy RNA
with a premature nonsense codon was 3.3 times shorter than that without a premature nonsense codon. Because the wild-type waxy
transcripts, which are derived from the Wxb
gene predominantly distributed among Japonica rice, have been shown to
be less efficiently spliced and their alternative splicing has been
documented, we examined whether these splicing properties influenced
the efficiency of NMD. However, no effects were observed. These results
established that NMD occurs in rice waxy RNA containing a premature nonsense codon.
*
Corresponding author; e-mail simamoto{at}bs.aist-nara.ac.jp;
fax 81-743-72-5509.
© 2001 American Society of Plant Physiologists
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