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Plant Physiol, March 2001, Vol. 125, pp. 1450-1458

Hydrogen Peroxide Mediates the Induction of Chloroplastic Ndh Complex under Photooxidative Stress in Barley1

Leonardo M. Casano,* Mercedes Martín, and Bartolomé Sabater

Departamento de Biología Vegetal, Universidad de Alcalá de Henares, 28871-Alcalá de Henares, Madrid, Spain

Chloroplast-encoded NDH polypeptides (components of the plastid Ndh complex) and the NADH dehydrogenase activity of the Ndh complex (NADH-DH) increased under photooxidative stress. The possible involvement of H2O2-mediated signaling in the photooxidative induction of chloroplastic ndh genes was thoroughly studied. We have analyzed the changes in the NADH-DH and steady-state levels of NDH-F polypeptide and ndhB and ndhF transcripts in barley (Hordeum vulgare cv Hassan) leaves. Subapical leaf segments were incubated in growing light (GL), photooxidative light (PhL), GL and H2O2 (GL + H2O2), or PhL and 50 nM paraquat in the incubation medium. Treatments with H2O2 under GL mimicked the photooxidative stimulus, causing a dose-dependent increase of NADH-DH and NDH-F polypeptide. The kinetic of Ndh complex induction was further studied in leaves pre-incubated with or without the H2O2-scavenger dimethyltiourea. NADH-DH and NDH-F polypeptide rapidly increased up to 16 h in PhL, GL+ H2O2, and, at higher rate, in PhL and paraquat. The observed increases of NADH-DH and NDH-F after 4 h in PhL and GL + H2O2 were not accompanied by significant changes in ndhB and ndhF transcripts. However, at 16-h incubations NADH-DH and NDH-F changes closely correlated with higher ndhB and ndhF transcript levels. All these effects were prevented by dimethylthiourea. It is proposed that the induction of chloroplastic ndh genes under photooxidative stress is mediated by H2O2 through mechanisms that involve a rapid translation of pre-existing transcripts and the increase of the ndh transcript levels.

1 This work was supported by the Spanish Dirección General de Investigacion Cientifica y Technica (grant no. PB96-0675) and by the Universidad de Alcalá (grant no. E011/2000).

* Corresponding author; e-mail leonardo.casano{at}uah.es; fax 34-91-885-5066.

© 2001 American Society of Plant Physiologists


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