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Plant Physiol, March 2001, Vol. 125, pp. 1485-1498
Regulation of Protein Degradation and Protease Expression by
Mannose in Maize Root Tips. Pi Sequestration by Mannose May Hinder the
Study of Its Signaling Properties
Renaud
Brouquisse,*
Adeline
Evrard,
Dominique
Rolin,
Philippe
Raymond, and
Claude
Roby
Unité de Physiologie Végétale, Institut National
de la Recherche Agronomique, CR de Bordeaux, BP 81, Villenave d'Ornon
cedex, France (R.B., P.R.); Commissariat à l'Energie Atomique
and Université Joseph Fourier, Laboratoire de Résonance
Magnétique en Biologie Métabolique, Département de
Biologie Moléculaire et Structurale, 17 rue des Martyrs, 38054 Grenoble cedex 9, France (A.E., C.R.); and Laboratoire du
Métabolisme des Plantes et de Résonance Magnétique,
Université de Bordeaux 2, Institut National de la
Recherche Agronomique, CR de Bordeaux, BP 81, Villenave d'Ornon
cedex, France (D.R.)
The effects of mannose (Man) and glucose (Glc) on central
metabolism, proteolysis, and expression of the root starvation-induced protease (RSIP; F. James, R. Brouquisse, C. Suire, A. Pradet, P. Raymond [1996] Biochem J 320: 283-292) were investigated in maize
(Zea mays L. cv DEA) root tips. Changes in metabolite
concentrations (sugars, ester-phosphates, adenine nucleotides, and
amino acids) were monitored using in vivo and in vitro 13C-
and 31P-NMR spectroscopy, in parallel with the changes in
respiration rates, protein contents, proteolytic activities, and RSIP
amounts. The inhibition of proteolysis, the decrease in proteolytic
activities, and the repression of RSIP expression triggered by Man, at
concentrations usually used to study sugar signaling (2 and 10 mM), were found to be related to a drop of energy
metabolism, primarily due to a Man-induced Pi sequestration. However,
when supplied at low concentration (2 mM) and with the
adequate phosphate concentration (30 mM), energy metabolism
was restored and Man repressed proteolysis similarly to Glc, when
provided at the same concentration. These results indicate that Man
should be used with caution as a Glc analog to study signalization by
sugars in plants because possible signaling effects may be hindered by
Pi sequestration.
*
Corresponding author; e-mail brouquis{at}bordeaux.inra.fr; fax
33-556-84-32-45.
© 2001 American Society of Plant Physiologists
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