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Plant Physiol, March 2001, Vol. 125, pp. 1508-1516
Expression of a Gibberellin 2-Oxidase Gene around the Shoot Apex
Is Related to Phase Transition in Rice1
Tomoaki
Sakamoto,
Masatomo
Kobayashi,*
Hironori
Itoh,
Akemi
Tagiri,
Toshiaki
Kayano,
Hiroshi
Tanaka,
Shuichi
Iwahori, and
Makoto
Matsuoka
Institute of Agriculture and Forestry, University of Tsukuba,
Tsukuba 305-8572, Japan (T.S., S.I.); RIKEN Tsukuba Institute, Tsukuba
305-0074, Japan (M.K.); BioScience Center, Nagoya University, Chikusa,
Nagoya 464-0814, Japan (H.I., M.M.); and Department of Biotechnology,
National Institute of Agrobiological Resources, Tsukuba 305-8602,
Japan (A.T., T.K., H.T.)
A major catabolic pathway for gibberellin (GA) is initiated by
2 -hydroxylation, a reaction catalyzed by GA 2-oxidase. We have
isolated and characterized a cDNA, designated Oryza
sativa GA 2-oxidase 1 (OsGA2ox1) from rice
(Oryza sativa L. cv Nipponbare) that encodes a GA
2-oxidase. The encoded protein, produced by heterologous expression in
Escherichia coli, converted GA1,
GA4, GA9, GA20, and
GA44 to the corresponding 2-hydroxylated products GA8, GA34, GA51, GA29,
and GA98, respectively. Ectopic expression of the
OsGA2ox1 cDNA in transgenic rice inhibited stem
elongation and the development of reproductive organs. These transgenic
plants were deficient in endogenous GA1. These results
indicate that OsGA2ox1 encodes a GA 2-oxidase, which is
functional not only in vitro but also in vivo. OsGA2ox1
was expressed in shoot apex and roots but not in leaves and stems. In
situ hybridization analysis revealed that OsGA2ox1 mRNA
was localized in a ring at the basal region of leaf primordia and young
leaves. This ring-shaped expression around the shoot apex was
drastically decreased after the phase transition from vegetative to
reproductive growth. It was absent in the floral meristem, but it was
still present in the lateral meristem that remained in the vegetative
phase. These observations suggest that OsGA2ox1 controls
the level of bioactive GAs in the shoot apical meristem; therefore,
reduction in its expression may contribute to the early development of
the inflorescence meristem.
1
This work was supported in part by a
Grant-in-Aid for Scientific Research from the Ministry of Education,
Science, Sports and Culture of Japan (grant no. 11306003 to S.I.); by
the Program for Promotion of Basic Research Activities for Innovation
Biosciences (grant to H.T. and M.M.); and by a research fellowship from
the Japan Society for the Promotion of Science (to T.S.).
*
Corresponding author; e-mail kobayasi{at}rtc.riken.go.jp; fax
81-298-36-9060.
© 2001 American Society of Plant Physiologists
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