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Plant Physiol, April 2001, Vol. 125, pp. 1603-1610
Cell-Type-Specific Expression of Plant Cytochrome c
mRNA in Developing Flowers and Roots1
Karina F.
Ribichich,
Mariana F.
Tioni,
Raquel L.
Chan, and
Daniel
H.
Gonzalez*
Cátedra de Biología Celular y Molecular, Facultad de
Bioquímica y Ciencias Biológicas, Universidad Nacional
del Litoral, CC 242 Paraje El Pozo, 3000 Santa Fe, Argentina
We have used RNA in situ hybridization to analyze the expression of
transcripts encoding cytochrome c in different tissues and organs of sunflower (Helianthus annuus). Although
northern-blot hybridization experiments indicate that the relative
abundance of transcripts does not vary greatly, we have detected
important changes in localization during flower development. Enhanced
expression is observed in floral meristems as soon as they are
discernible from the central portion of the capitulum containing the
inflorescence meristem. As flowers develop, labeling is observed in all
developing floral organ primordia. Later in development, expression in
petals is reduced, and only the central portion of the flower becomes labeled. During the process of stamen formation, hybridization signals
were obtained mainly in anthers. Less developed flowers at this stage
showed expression through the archesporial tissue. During meiosis, the
label was observed mainly in tapetal cells. Specific expression
patterns, similar to those obtained for sunflower, were observed when
Arabidopsis flowers were analyzed with a homologous cytochrome
c probe. Specific patterns of expression were also observed in young sunflower roots. In this case, enhanced expression was detected in developing endodermis and pericycle and in protoxylem initials. We conclude that cell-specific mechanisms operate to regulate
the abundance of cytochrome c encoding transcripts in different plant tissues. The overlap between the expression patterns of
the nuclear encoded cytochrome c gene and some
mitochondrial genes suggests the existence of coordinated mechanisms of expression.
1
This work was supported by the Consejo Nacional
de Investigaciones Científicas y Técnicas, Agencia
Nacional de Promoción Científica y Tecnológica, and
Fundacion Antorchas (Argentina). R.L.C. and D.H.G. are members of
Consejo Nacional de Investigaciones Científicas y
Técnicas, and M.F.T. is a fellow of the same institution.
*
Corresponding author; e-mail dhgonza{at}fbcb.unl.edu.ar; fax
54-342-4575219.
© 2001 American Society of Plant Physiologists
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