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Plant Physiol, April 2001, Vol. 125, pp. 1643-1654
Salt-Induced Expression of the Vacuolar H+-ATPase in
the Common Ice Plant Is Developmentally Controlled and Tissue
Specific1
Dortje
Golldack and
Karl-Josef
Dietz*
Department of Physiology and Biochemistry of Plants, Faculty of
Biology, University of Bielefeld, D-33501 Bielefeld, Germany
For salinity stress tolerance in plants, the vacuolar type
H+-ATPase (V-ATPase) is of prime importance in energizing
sodium sequestration into the central vacuole and it is known to
respond to salt stress with increased expression and enzyme activity. In this work we provide information that the expressional response to
salinity of the V-ATPase is regulated tissue and cell specifically under developmental control in the facultative halophyte common ice
plant (Mesembryanthemum crystallinum). By transcript
analysis of subunit E of the V-ATPase, amounts did not change in
response to salinity stress in juvenile plants that are not
salt-tolerant. In a converse manner, in halotolerant mature plants the
transcript levels increased in leaves, but not in roots when salt
stressed for 72 h. By in situ hybridizations and immunocytological
protein analysis, subunit E was shown to be synthesized in all cell
types. During salt stress, signal intensity declined in root cortex
cells and in the cells of the root vascular cylinder. In salt-stressed leaves of mature plants, the strongest signals were localized surrounding the vasculature. Within control cells and with highest abundance in mesophyll cells of salt-treated leaves, accumulation of
subunit E protein was observed in the cytoplasm, indicating its
presence not only in the tonoplast, but also in other endoplasmic compartments.
1
This work was supported by the Deutsche
Forschungsgemeinschaft within the Sonderforschungsbereich 176.
*
Corresponding author; e-mail
karl-josef.dietz{at}biologie.uni-bielefeld.de; fax
49-521-106-6039.
© 2001 American Society of Plant Physiologists
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