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Plant Physiol, April 2001, Vol. 125, pp. 1655-1666
Developmental and Stress Regulation of RCI2A and
RCI2B, Two Cold-Inducible Genes of Arabidopsis Encoding
Highly Conserved Hydrophobic Proteins1
Joaquín
Medina,
Rafael
Catalá, and
Julio
Salinas*
Departamento de Mejora Genética y Biotecnología,
Instituto Nacional de Investigación y Tecnología
Agraria y Alimentaria (INIA), Carretera de la Coruña, Km. 7, 28040 Madrid, Spain
The capability of most higher plants to tolerate environmental
conditions strongly depends on their developmental stage. In addition,
environmental factors have pleiotropic effects on many developmental
processes. The interaction between plant development and environmental
conditions implies that some genes must be regulated by both
environmental factors and developmental cues. To understand their
developmental regulation and obtain possible clues on their functions,
we have isolated genomic clones for RCI2A and
RCI2B, two genes from Arabidopsis ecotype Columbia
(Col), whose expression is induced in response to low temperature,
dehydration, salt stress, and abscisic acid. The promoters of
RCI2A and RCI2B were fused to the
uidA (GUS)-coding sequence and the
resulting constructs used to transform Arabidopsis. GUS activity was
analyzed in transgenic plants during development under both stressed
and unstressed conditions. Transgenic plants with either the
RCI2A or RCI2B promoter showed strong
GUS expression during the first stages of seed
development and germination, in vascular bundles, pollen, and most
interestingly in guard cells. When transgenic plants were exposed to
low temperature, dehydration, salt stress, or abscisic acid, reporter
gene expression was induced in most tissues. These results indicate
that RCI2A and RCI2B are regulated at
transcriptional level during plant development and in response to
different environmental stimuli and treatments. The potential role of
RCI2A and RCI2B in plant development and
stress response is discussed.
1
This work was supported by the European Union
(grant no. BIO CT96-0101), by Comision Interministerial de Ciencia y
Tecnologia (grant no. BIO98-0189), by a fellowship from de Comunidad
Autónoma de Madrid (to J.M.), and by a fellowship from Instituto
Nacional de Investigacion y Tecnologia Agraria y Alimentaria (to
R.C.).
*
Corresponding author; e-mail salinas{at}inia.es; fax
34-91- 357-3107.
© 2001 American Society of Plant Physiologists
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