|
Plant Physiol, April 2001, Vol. 125, pp. 1667-1678
Tuber Physiology and Properties of Starch from Tubers of
Transgenic Potato Plants with Altered Plastidic Adenylate Transporter
Activity1
Peter
Geigenberger,*
Claudia
Stamme,
Joachim
Tjaden,
Alexander
Schulz,
Paul W.
Quick,
Thomas
Betsche,
H. J.
Kersting, and
H.
Ekkehard
Neuhaus
Botanisches Institut der Universität Heidelberg, Im
Neuenheimer Feld 360, D-69120 Heidelberg, Germany (P.G.);
Pflanzenphysiologie, Universität Kaiserslautern,
Erwin-Schrödinger-Strasse, D-67653 Kaiserslautern, Germany
(C.S., J.T., H.E.N.); The Royal Veterinary and Agricultural University,
Department of Plant Biology, 40 Thorvaldsenvej, DK-1871 Frederiksberg
C, Copenhagen, Denmark (A.S.); University of Sheffield, Department of
Animal and Plant Sciences, P.O. Box 601, Sheffield S10 2UQ, United
Kingdom (P.W.Q); and Bundesforschungsanstalt für Getreide-,
Kartoffel-, und Fettforschung, Schützenberg 12, D-32756 Detmold,
Germany (T.B., H.J.K.)
We showed recently that antisense plants with decreased activity of
the plastidic ATP/ADP-transporter protein exhibit drastically reduced
levels of starch and a decreased amylose/amylopectin ratio, whereas
sense plants with increased activity of the transporter possessed more
starch than wild-type plants and an increased amylose/amylopectin ratio. In this paper we investigate the effect of altered plastidic ATP/ADP-transporter protein expression on primary metabolism and granule morphology in more detail. Tuber tissues from antisense and
sense plants exhibited substantially increased respiratory activity
compared with the wild type. Tubers from antisense plants contained
markedly increased levels of free sugars, UDP-Glc, and hexose
phosphates, whereas phosphoenolpyruvate, isocitrate,
ATP, ADP, AMP, UTP, UDP, and inorganic pyrophosphate levels were
slightly decreased. In contrast, tubers from sense plants revealed a
slight increase in adenine and uridine nucleotides and in the levels of
inorganic pyrophosphate, whereas no significant changes in the levels
of soluble sugars and metabolites were observed. Antisense tubers
contained 50% reduced levels of ADP-Glc, whereas sense tubers
contained up to 2-fold increased levels of this sole precursor for
starch biosynthesis. Microscopic examination of starch grain morphology
revealed that the size of starch grains from antisense tubers was
substantially smaller (50%) compared with the wild type. The large
starch grains from sense tubers appeared of a more angular morphology,
which differed to the more ellipsoid shape of wild type grains. The
results suggest a close interaction between plastidial adenylate
transport and starch biosynthesis, indicating that ADP-Glc
pyrophosphorylase is ATP-limited in vivo and that changes in ADP-Glc
concentration determine starch yield, as well as granule morphology.
Possible factors linking starch synthesis and respiration are discussed.
1
This work was supported by the
Sonderforschunsgbereich 431 (Teilprojekt K7 to H.E.N.), by the Fonds
der Chemischen Industrie (grant to H.E.N.), and by the Deutsche
Forschungsgemeinschaft (grant to P.G.).
*
Corresponding author; e-mail
pgeig{at}mail.bot.uni-heidelberg.de; fax 49-6221-545859.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
Y. S. Nagai, C. Sakulsingharoj, G. E. Edwards, H. Satoh, T. W. Greene, B. Blakeslee, and T. W. Okita
Control of Starch Synthesis in Cereals: Metabolite Analysis of Transgenic Rice Expressing an Up-Regulated Cytoplasmic ADP-Glucose Pyrophosphorylase in Developing Seeds
Plant Cell Physiol.,
March 1, 2009;
50(3):
635 - 643.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Schlegel, O. Mirus, A. von Haeseler, and E. Schleiff
The Tetratricopeptide Repeats of Receptors Involved in Protein Translocation across Membranes
Mol. Biol. Evol.,
December 1, 2007;
24(12):
2763 - 2774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Malone, V. Mittova, R. G. Ratcliffe, and N. J. Kruger
The Response of Carbohydrate Metabolism in Potato Tubers to Low Temperature
Plant Cell Physiol.,
September 1, 2006;
47(9):
1309 - 1322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Colasante, V. P. Alibu, S. Kirchberger, J. Tjaden, C. Clayton, and F. Voncken
Characterization and Developmentally Regulated Localization of the Mitochondrial Carrier Protein Homologue MCP6 from Trypanosoma brucei.
Eukaryot. Cell,
August 1, 2006;
5(8):
1194 - 1205.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Leroch, S. Kirchberger, I. Haferkamp, M. Wahl, H. E. Neuhaus, and J. Tjaden
Identification and Characterization of a Novel Plastidic Adenine Nucleotide Uniporter from Solanum tuberosum
J. Biol. Chem.,
May 6, 2005;
280(18):
17992 - 18000.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Reiser, N. Linka, L. Lemke, W. Jeblick, and H. E. Neuhaus
Molecular Physiological Analysis of the Two Plastidic ATP/ADP Transporters from Arabidopsis
Plant Physiology,
November 1, 2004;
136(3):
3524 - 3536.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. P. M. Weber, J. Schneidereit, and L. M. Voll
Using mutants to probe the in vivo function of plastid envelope membrane metabolite transporters
J. Exp. Bot.,
June 1, 2004;
55(400):
1231 - 1244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Vojta, M. Alavi, T. Becker, F. Hormann, M. Kuchler, J. Soll, R. Thomson, and E. Schleiff
The Protein Translocon of the Plastid Envelopes
J. Biol. Chem.,
May 14, 2004;
279(20):
21401 - 21405.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Vigeolas, J. T. van Dongen, P. Waldeck, D. Huhn, and P. Geigenberger
Lipid Storage Metabolism Is Limited by the Prevailing Low Oxygen Concentrations within Developing Seeds of Oilseed Rape
Plant Physiology,
December 1, 2003;
133(4):
2048 - 2060.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. L. Bologa, A. R. Fernie, A. Leisse, M. Ehlers Loureiro, and P. Geigenberger
A Bypass of Sucrose Synthase Leads to Low Internal Oxygen and Impaired Metabolic Performance in Growing Potato Tubers
Plant Physiology,
August 1, 2003;
132(4):
2058 - 2072.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Pilling and A. M. Smith
Growth Ring Formation in the Starch Granules of Potato Tubers
Plant Physiology,
May 1, 2003;
132(1):
365 - 371.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Geigenberger
Regulation of sucrose to starch conversion in growing potato tubers
J. Exp. Bot.,
January 3, 2003;
54(382):
457 - 465.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Emes, C. G. Bowsher, C. Hedley, M. M. Burrell, E. S. F. Scrase-Field, and I. J. Tetlow
Starch synthesis and carbon partitioning in developing endosperm
J. Exp. Bot.,
January 3, 2003;
54(382):
569 - 575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Tiessen, J. H. M. Hendriks, M. Stitt, A. Branscheid, Y. Gibon, E. M. Farre, and P. Geigenberger
Starch Synthesis in Potato Tubers Is Regulated by Post-Translational Redox Modification of ADP-Glucose Pyrophosphorylase: A Novel Regulatory Mechanism Linking Starch Synthesis to the Sucrose Supply
PLANT CELL,
September 1, 2002;
14(9):
2191 - 2213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Linke, U. Conrath, W. Jeblick, T. Betsche, A. Mahn, K. During, and H. E. Neuhaus
Inhibition of the Plastidic ATP/ADP Transporter Protein Primes Potato Tubers for Augmented Elicitation of Defense Responses and Enhances Their Resistance against Erwinia carotovora
Plant Physiology,
August 1, 2002;
129(4):
1607 - 1615.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
