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Plant Physiol, April 2001, Vol. 125, pp. 1766-1777
Genetic Analysis of Amino Acid Accumulation in
opaque-2 Maize Endosperm1
Xuelu
Wang and
Brian A.
Larkins*
Department of Plant Sciences, University of Arizona, Tucson,
Arizona 85721
The opaque-2 mutation in maize (Zea
mays) is associated with an increased level of free
amino acids (FAA) in the mature endosperm. In particular, there is a
high concentration of lysine, the most limiting essential amino acid.
To investigate the basis for the high-FAA phenotype of
opaque-2 maize, we characterized amino acid accumulation
during endosperm development of several wild-type and
opaque-2 inbreds. Oh545o2 was found to
have an exceptionally high level of FAA, in particular those derived
from aspartate (Asp) and intermediates of glycolysis. The FAA content
in Oh545o2 is 12 times greater than its wild-type
counterpart, and three and 10 times greater than in
Oh51Ao2 and W64Ao2, respectively. We
crossed Oh545o2 to Oh51Ao2 and analyzed
the F2:3 progeny to identify genetic loci linked with the
high FAA level in these mutants. Quantitative trait locus mapping
identified four significant loci that account for about 46% of the
phenotypic variance. One locus on the long arm of chromosome 2 is
coincident with genes encoding a monofunctional Asp kinase 2 and a
bifunctional Asp kinase-homo-Ser dehydrogenase-2, whereas another locus
on the short arm of chromosome 3 is linked with a cytosolic triose
phosphate isomerase 4. The results suggest an alternation of amino acid and carbon metabolism leads to overproduction and accumulation of FAA
in opaque-2 mutants.
1
This research was supported by Pioneer Hi-Bred
(grant to B.A.L.).
*
Corresponding author; e-mail Larkins{at}Ag.Arizona.edu; fax
520-621-3692.
© 2001 American Society of Plant Physiologists
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