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Plant Physiol, April 2001, Vol. 125, pp. 1813-1820

ANT1, an Aromatic and Neutral Amino Acid Transporter in Arabidopsis

Lishan Chen, Adriana Ortiz-Lopez, Alan Jung, and Daniel R. Bush*

Program in Physiological and Molecular Plant Biology (L.C., D.R.B.), Photosynthesis Research Unit, United States Department of Agriculture-Agricultural Research Service (A.O.-L., D.R.B.), and Department of Plant Biology (A.J., D.R.B.), University of Illinois, 196 E.R. Madigan Laboratory, 1201 W. Gregory Drive, Urbana, Illinois 61801

A new amino acid transporter was identified from the Arabidopsis expressed sequence tag cDNAs by expressing the cDNA in a yeast amino acid transport mutant. Transport analysis of the expressed protein in yeast showed that it transports aromatic and neutral amino acids, as well as arginine. This transporter (ANT1, aromatic and neutral transporter) also transports indole-3-acetic acid and 2,4-dichlorophenoxyacetic acid. The cDNA is 1.6 kb in length with an open reading frame that codes for a protein with 432 amino acids and a calculated molecular mass of 50 kD. Hydropathy analysis showed ANT1 is an integral membrane protein with 11 putative membrane-spanning domains. Southern analysis and a BLAST search of the Arabidopsis genome database suggests that ANT1 is part of a small gene family containing at least five members. Phylogenetic comparisons with other known amino acid transporters in plants suggests that ANT1 represents a new class of amino acid transporter. RNA gel-blot analysis showed that this transporter is expressed in all organs with highest abundance in flowers and cauline leaves.

* Corresponding author; e-mail dbush{at}uiuc.edu; fax 217-244-4419.

© 2001 American Society of Plant Physiologists


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