|
Plant Physiol, April 2001, Vol. 125, pp. 1821-1830
Isolation of a CONSTANS Ortholog from
Pharbitis nil and Its Role in
Flowering1
Jiayou
Liu,2
Jianping
Yu,
Lee
McIntosh,
Hans
Kende, and
Jan A.D.
Zeevaart*
Departments of Energy Plant Research Laboratory (J.L., J.Y., H.K.,
L.M., J.A.D.Z.), Biochemistry and Molecular Biology (L.M.), and Plant
Biology (H.K., J.A.D.Z.), Michigan State University, East Lansing,
Michigan 48824-1312
The short-day plant Pharbitis nil is a model plant
for the study of photoperiodic control of floral initiation. Flower
formation can be induced at the cotyledon stage by a single long night
of at least 14 h in duration. Using differential display of mRNA we identified a P. nil ortholog of the Arabidopsis
CONSTANS (CO) gene, which will be
referred to as PnCO. Expression of PnCO
was high after a 14-h night, but low when the dark period was 12 h or less. Our results indicate that the level of the PnCO
transcript is photoperiodically regulated. After transfer from
continuous light to darkness, PnCO showed a circadian
pattern of expression. Expression of the CAB gene, which
is a molecular marker for the circadian clock, exhibited a different
pattern of expression than did PnCO and was not subject
to the same photoperiodic control. A major portion of the
PnCO transcripts contained an unspliced intron. Only the
intron-free PnCO was able to complement the
co mutant of Arabidopsis by shortening the time to
flower.
1
This work was supported by the U.S. Department
of Energy (grant no. De-FG02-91ER20021).
2
Present address: Department of Pathology, Wayne State
University, Detroit, MI 48201.
*
Corresponding author; e-mail zeevaart{at}msu.edu; fax
517-353-9168.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
C. C. Zheng, D. Potter, and S. D. O'Neill
Phytochrome gene expression and phylogenetic analysis in the short-day plant Pharbitis nil (Convolvulaceae): Differential regulation by light and an endogenous clock
Am. J. Botany,
July 1, 2009;
96(7):
1319 - 1336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Y. P. Chia, A. Muller, C. Jung, and E. S. Mutasa-Gottgens
Sugar beet contains a large CONSTANS-LIKE gene family including a CO homologue that is independent of the early-bolting (B) gene locus
J. Exp. Bot.,
July 1, 2008;
59(10):
2735 - 2748.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Hayama, B. Agashe, E. Luley, R. King, and G. Coupland
A Circadian Rhythm Set by Dusk Determines the Expression of FT Homologs and the Short-Day Photoperiodic Flowering Response in Pharbitis
PLANT CELL,
October 1, 2007;
19(10):
2988 - 3000.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Wenkel, F. Turck, K. Singer, L. Gissot, J. Le Gourrierec, A. Samach, and G. Coupland
CONSTANS and the CCAAT Box Binding Complex Share a Functionally Important Domain and Interact to Regulate Flowering of Arabidopsis
PLANT CELL,
November 1, 2006;
18(11):
2971 - 2984.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Thomson, J. D. Edwards, E. M. Septiningsih, S. E. Harrington, and S. R. McCouch
Substitution Mapping of dth1.1, a Flowering-Time Quantitative Trait Locus (QTL) Associated With Transgressive Variation in Rice, Reveals Multiple Sub-QTL
Genetics,
April 1, 2006;
172(4):
2501 - 2514.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Ishikawa, S. Tamaki, S. Yokoi, N. Inagaki, T. Shinomura, M. Takano, and K. Shimamoto
Suppression of the Floral Activator Hd3a Is the Principal Cause of the Night Break Effect in Rice
PLANT CELL,
December 1, 2005;
17(12):
3326 - 3336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Hayama and G. Coupland
The Molecular Basis of Diversity in the Photoperiodic Flowering Responses of Arabidopsis and Rice
Plant Physiology,
June 1, 2004;
135(2):
677 - 684.
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-J. Kim, J. Moon, I. Lee, J. Maeng, and S.-R. Kim
Molecular cloning and expression analysis of a CONSTANS homologue, PnCOL1, from Pharbitis nil
J. Exp. Bot.,
August 1, 2003;
54(389):
1879 - 1887.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Griffiths, R. P. Dunford, G. Coupland, and D. A. Laurie
The Evolution of CONSTANS-Like Gene Families in Barley, Rice, and Arabidopsis
Plant Physiology,
April 1, 2003;
131(4):
1855 - 1867.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F. Martinez-Garcia, A. Virgos-Soler, and S. Prat
Control of photoperiod-regulated tuberization in potato by the Arabidopsis flowering-time gene CONSTANS
PNAS,
November 12, 2002;
99(23):
15211 - 15216.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Mouradov, F. Cremer, and G. Coupland
Control of Flowering Time: Interacting Pathways as a Basis for Diversity
PLANT CELL,
May 1, 2002;
14(90001):
S111 - 130.
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. G. Simpson and C. Dean
Arabidopsis, the Rosetta Stone of Flowering Time?
Science,
April 12, 2002;
296(5566):
285 - 289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Yano, S. Kojima, Y. Takahashi, H. Lin, and T. Sasaki
Genetic Control of Flowering Time in Rice, a Short-Day Plant
Plant Physiology,
December 1, 2001;
127(4):
1425 - 1429.
[Full Text]
[PDF]
|
|
|
|
|
