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Plant Physiol, April 2001, Vol. 125, pp. 1854-1869
Light-Dependent Osmoregulation in Pea Stem Protoplasts.
Photoreceptors, Tissue Specificity, Ion Relationships, and
Physiological Implications
Cheng
Long1 and
Moritoshi
Iino*
Botanical Gardens, Graduate School of Science, Osaka City
University, Kisaichi, Katano-shi, Osaka 576-0004, Japan
Light-induced changes in the volume of protoplasts bathed in a
medium of constant osmolarity are useful indications of light-dependent cellular osmoregulation. With this in mind, we investigated the effect
of light on the volume of protoplasts isolated from the elongating
stems of pea (Pisum sativum) seedlings raised under red
light. The protoplasts were isolated separately from epidermal peels
and the remaining peeled stems. Under continuous red light, the
protoplasts of peeled stems swelled steadily, but those of epidermal
peels maintained a constant volume. Experiments employing far-red light
and phytochrome-deficient mutants revealed that the observed swelling
is a light-induced response mediated mainly by phytochromes A and B
with a little greater contribution by phytochrome A. Protoplasts of
epidermal peels and peeled stems shrank transiently in response to a
pulse of blue light. The blue light responsiveness in this shrinking
response, which itself is probably mediated by cryptochrome, is under
the strict control of phytochromes A and B with equal contributions by
these phytochromes. We suggest that the swelling response participates
in the maintenance of high tissue tension of elongating stems and that
the shrinking response is involved in stem growth inhibition. Other
findings include the following: The swelling is caused by uptake of
K+ and Cl . The presence of Ca2+
in the bathing medium is required for phytochrome signaling in the
swelling response, but not in the response establishing blue light
responsiveness. Phytochrome A mediates the two responses in a totally
red/far-red light reversible manner, as does phytochrome B.
1
Present address: Department of Biology, East
China Normal University, Shanghai 200062, People's Republic of China.
*
Corresponding author; e-mail iino{at}sci.osaka-cu.ac.jp; fax
81-72-891-7199.
© 2001 American Society of Plant Physiologists
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