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Plant Physiol, April 2001, Vol. 125, pp. 1919-1929
Increased Sensitivity of Photosynthesis to Antimycin A Induced by
Inactivation of the Chloroplast ndhB Gene. Evidence for
a Participation of the NADH-Dehydrogenase Complex to Cyclic Electron
Flow around Photosystem I1
Thierry
Joët,
Laurent
Cournac,
Eva M.
Horvath,2
Peter
Medgyesy,3 and
Gilles
Peltier*
Commissariat à l'Energie Atomique, Cadarache, Laboratoire
d'Ecophysiologie de la Photosynthèse, Département
d'Ecophysiologie Végétale et Microbiologie, Bât.
161, F-13108 Saint-Paul-lez-Durance, France (T.J., L.C., G.P.); and
Biological Research Center, Hungarian Academy of Science, P.O. Box 521, H-6701 Szeged, Hungary (E.M.H., P.M.)
Tobacco (Nicotiana tabacum var Petit Havana)
ndhB-inactivated mutants
(ndhB ) obtained by plastid transformation
(E.M. Horvath, S.O. Peter, T. Joët, D. Rumeau, L. Cournac, G.V.
Horvath, T.A. Kavanagh, C. Schäfer, G. Peltier, P. MedgyesyHorvath [2000] Plant Physiol 123: 1337-1350) were used to
study the role of the NADH-dehydrogenase complex (NDH) during
photosynthesis and particularly the involvement of this complex in
cyclic electron flow around photosystem I (PSI). Photosynthetic
activity was determined on leaf discs by measuring CO2
exchange and chlorophyll fluorescence quenchings during a dark-to-light
transition. In the absence of treatment, both non-photochemical and
photochemical fluorescence quenchings were similar in
ndhB and wild type (WT). When leaf
discs were treated with 5 µM antimycin A, an inhibitor of
cyclic electron flow around PSI, both quenchings were strongly
affected. At steady state, maximum photosynthetic electron transport
activity was inhibited by 20% in WT and by 50% in
ndhB. Under non-photorespiratory
conditions (2% O2, 2,500 µL L1
CO2), antimycin A had no effect on photosynthetic activity
of WT, whereas a 30% inhibition was observed both on quantum yield of
photosynthesis assayed by chlorophyll fluorescence and on
CO2 assimilation in ndhB. The
effect of antimycin A on ndhB could not be
mimicked by myxothiazol, an inhibitor of the mitochondrial cytochrome
bc1 complex, therefore showing that it is
not related to an inhibition of the mitochondrial electron transport
chain but rather to an inhibition of cyclic electron flow around PSI. We conclude to the existence of two different pathways of cyclic electron flow operating around PSI in higher plant chloroplasts. One of
these pathways, sensitive to antimycin A, probably involves ferredoxin
plastoquinone reductase, whereas the other involves the NDH complex.
The absence of visible phenotype in ndhB
plants under normal conditions is explained by the complement of these
two pathways in the supply of extra-ATP for photosynthesis.
1
This work was supported by the European
Community Biotechnology program (grant no. BIO4-CT-97-2245).
2
Present address: Department of Genetics, Trinity
College, University of Dublin, Dublin 2, Ireland.
3
Present address: Department of Biology, National
University of Ireland, Maynooth, County Kildare, Ireland.
*
Corresponding author: e-mail gilles.peltier{at}cea.fr; fax
33-4-42256265.
© 2001 American Society of Plant Physiologists
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