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Plant Physiol, April 2001, Vol. 125, pp. 1941-1948

Expression of D-myo-Inositol-3-Phosphate Synthase in Soybean. Implications for Phytic Acid Biosynthesis1

Carla E. Hegeman,2 Laura L. Good, and Elizabeth A. Grabau*

Department of Plant Pathology, Physiology, and Weed Science, Fralin Biotechnology Center, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061-0346

Phytic acid, a phosphorylated derivative of myo-inositol, functions as the major storage form of phosphorus in plant seeds. Myo-inositol phosphates, including phytic acid, play diverse roles in plants as signal transduction molecules, osmoprotectants, and cell wall constituents. D-myo-inositol-3-phosphate synthase (MIPS EC 5.5.1.4) catalyzes the first step in de novo synthesis of myo-inositol. A soybean (Glycine max) MIPS cDNA (GmMIPS1) was isolated by reverse transcriptase-PCR using consensus primers designed from highly conserved regions in other plant MIPS sequences. Southern-blot analysis and database searches indicated the presence of at least four MIPS genes in the soybean genome. Northern-blot and immunoblot analyses indicated higher MIPS expression and accumulation in immature seeds than in other soybean tissues. MIPS was expressed early in the cotyledonary stage of seed development. The GmMIPS1 expression pattern suggested that it encodes a MIPS isoform that functions in seeds to generate D-myo-inositol-3-phosphate as a substrate for phytic acid biosynthesis.

1 This work was supported in part by the U.S. Department of Agriculture National Research Initiative Competitive Grants Program (grant no. 97-35504-4997).

2 Present address: Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853.

* Corresponding author; e-mail egrabau{at}vt.edu; fax 540- 231-7126.

© 2001 American Society of Plant Physiologists


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