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Plant Physiol, April 2001, Vol. 125, pp. 1988-2000
Regulation of psbA and psaE Expression by
Light Quality in Synechocystis Species PCC 6803. A
Redox Control Mechanism
Kamal El
Bissati and
Diana
Kirilovsky*1
Unité Mixte de Recherche 8543, Centre National de la
Recherche Scientifique, Ecole Normale Supérieure, 46 rue
d'Ulm, 75230 Paris, France
We investigated the influence of light of different wavelengths on
the expression of the psbA gene, which encodes the D1
protein of the photosystem II and the psaE gene, which
encodes the subunit Psa-E of the photosystem I, in
Synechocystis sp PCC 6803. In an attempt to
differentiate between a light-sensory and a redox-sensory signaling
processes, the effect of orange, blue, and far-red light was studied in
the wild-type and in a phycobilisome-less mutant. Transferring
wild-type cells from one type of illumination to another induced
changes in the redox state of the electron transport chain and in
psbA and psaE expression. Blue and
far-red lights (which are preferentially absorbed by the photosystem I)
induced an accumulation of psbA transcripts and a
decrease of the psaE mRNA level. In contrast, orange
light (which is preferentially absorbed by the photosystem II) induced
a large accumulation of psaE transcripts and a decrease
of psbA mRNA level. Transferring mutant cells from blue
to orange light (or vice versa) had no effect either on the redox state
of the electron transport chain or on the levels of psbA
and psaE mRNAs. Thus, light quality seems to regulate
expression of these genes via a redox sensory mechanism in
Synechocystis sp PCC 6803 cells. Our data suggest that
the redox state of one of the electron carriers between the
plastoquinone pool and the photosystem I has opposite influences on
psbA and psaE expression. Its reduction
induces accumulation of psaE transcripts, and its
oxidation induces accumulation of psbA mRNAs.
1
Present address: Unité de
Recherche Associée 2096, Centre National de la Recherche
Scientifique/Commissariat à l'Energie Atomique,
Département de Biologie Cellulaire et Moléculaire, Section de Bioénergétique, Bât 532, Commissariat
à l'Energie Atomique Saclay, 91191 Gif sur Yvette, France.
*
Corresponding author; e-mail kirilovs{at}dsvidf.cea.fr; fax
33-1-69-08-87-17.
© 2001 American Society of Plant Physiologists
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