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Plant Physiol, April 2001, Vol. 125, pp. 2007-2015
Active Oxygen Produced during Selective Excitation of Photosystem
I Is Damaging Not Only to Photosystem I, But Also to Photosystem
II1
Staffan Erling
Tjus,
Henrik Vibe
Scheller,*
Bertil
Andersson, and
Birger Lindberg
Møller
Plant Biochemistry Laboratory, Department of Plant Biology, The
Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Copenhagen, Denmark (S.E.T., H.V.S., B.L.M.);
Department of Biochemistry, Stockholm University, SE-10609 Stockholm,
Sweden (S.E.T., B.A.); and Division of Cell Biology,
Linköping University, SE-58185 Linköping, Sweden
(B.A.)
With the aim to specifically study the molecular mechanisms behind
photoinhibition of photosystem I, stacked spinach (Spinacia oleracea) thylakoids were irradiated at 4°C with far-red
light (>715 nm) exciting photosystem I, but not photosystem II.
Selective excitation of photosystem I by far-red light for 130 min
resulted in a 40% inactivation of photosystem I. It is surprising that this treatment also caused up to 90% damage to photosystem II. This
suggests that active oxygen produced at the reducing side of
photosystem I is highly damaging to photosystem II. Only a small pool
of the D1-protein was degraded. However, most of the D1-protein was
modified to a slightly higher molecular mass, indicative of a
damage-induced conformational change. The far-red illumination was also
performed using destacked and randomized thylakoids in which the
distance between the photosystems is shorter. Upon 130 min of
illumination, photosystem I showed an approximate 40% inactivation as
in stacked thylakoids. In contrast, photosystem II only showed 40%
inactivation in destacked and randomized thylakoids, less than one-half
of the inactivation observed using stacked thylakoids. In accordance
with this, photosystem II, but not photosystem I is more protected from
photoinhibition in destacked thylakoids. Addition of active oxygen
scavengers during the far-red photosystem I illumination demonstrated
superoxide to be a major cause of damage to photosystem I, whereas
photosystem II was damaged mainly by superoxide and hydrogen peroxide.
1
This work was supported by the Nordic Joint
Committee for Agricultural Research.
*
Corresponding author; e-mail hvs{at}kvl.dk; fax 45-35283333.
© 2001 American Society of Plant Physiologists
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