|
Plant Physiol, April 2001, Vol. 125, pp. 2059-2067
Expression of a Pseudomonas aeruginosa Citrate
Synthase Gene in Tobacco Is Not Associated with Either Enhanced Citrate
Accumulation or Efflux1
Emmanuel
Delhaize,*
Diane M.
Hebb, and
Peter R.
Ryan
Commonwealth Scientific and Industrial Research Organization Plant
Industry, G.P.O. Box 1600, Canberra Australian Capital Territory 2601, Australia
Aluminum (Al) toxicity and poor phosphorus (P) availability are
factors that limit plant growth on many agricultural soils. Previous
work reported that expression of a Pseudomonas
aeruginosa citrate synthase gene in tobacco (Nicotiana
tabacum; CSb lines) resulted in improved Al tolerance (J.M. de
la Fuente, V. Ramírez-Rodríguez, J.L. Cabrera-Ponce, L. Herrera-Estrella [1997] Science 276: 1566-1568) and an enhanced
ability to acquire P from alkaline soils (J. López-Bucio, O. Martínez de la Vega, A. Guevara-García, L. Herrera-Estrella [2000] Nat Biotechnol 18: 450-453). These effects
were attributed to the P. aeruginosa citrate synthase
increasing the biosynthesis and efflux of citrate from roots. To verify
these findings we: (a) characterized citrate efflux from roots of
wild-type tobacco; (b) generated tobacco lines expressing the citrate
synthase gene from P. aeruginosa; and (c) analyzed
selected CSb lines described above. Al stimulated citrate efflux from
intact roots of wild-type tobacco and root apices were found to be
responsible for most of the efflux. Despite generating transgenic
tobacco lines that expressed the citrate synthase protein at up to a
100-fold greater level than the previously described CSb lines, these
lines did not show increased accumulation of citrate in roots or
increased Al-activated efflux of citrate from roots. Selected CSb
lines, similarly, failed to show differences compared with controls in either citrate accumulation or efflux. We conclude that expression of
the P. aeruginosa citrate synthase gene in plants is
unlikely to be a robust and easily reproducible strategy for enhancing the Al tolerance and P-nutrition of crop and pasture species.
1
This work was supported by the Australian Grains
Research and Development Corporation.
*
Corresponding author; e-mail e.delhaize{at}pi.csiro.au; fax
61-2-6246-5000.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
A. Sienkiewicz-Porzucek, A. Nunes-Nesi, R. Sulpice, J. Lisec, D. C. Centeno, P. Carillo, A. Leisse, E. Urbanczyk-Wochniak, and A. R. Fernie
Mild Reductions in Mitochondrial Citrate Synthase Activity Result in a Compromised Nitrate Assimilation and Reduced Leaf Pigmentation But Have No Effect on Photosynthetic Performance or Growth
Plant Physiology,
May 1, 2008;
147(1):
115 - 127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. YANG, L. ZHANG, Y. Y. LI, J. F. YOU, P. WU, and S. J. ZHENG
Citrate Transporters Play a Critical Role in Aluminium-stimulated Citrate Efflux in Rice Bean (Vigna umbellata) Roots
Ann. Bot.,
April 1, 2006;
97(4):
579 - 584.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Yang, S. J. Zheng, Y. F. He, and H. Matsumoto
Aluminium resistance requires resistance to acid stress: a case study with spinach that exudes oxalate rapidly when exposed to Al stress
J. Exp. Bot.,
April 1, 2005;
56(414):
1197 - 1203.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Delhaize, P. R. Ryan, D. M. Hebb, Y. Yamamoto, T. Sasaki, and H. Matsumoto
Engineering high-level aluminum tolerance in barley with the ALMT1 gene
PNAS,
October 19, 2004;
101(42):
15249 - 15254.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. HAMMOND, M. R. BROADLEY, and P. J. WHITE
Genetic Responses to Phosphorus Deficiency
Ann. Bot.,
September 1, 2004;
94(3):
323 - 332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Ermolayev, W. Weschke, and R. Manteuffel
Comparison of Al-induced gene expression in sensitive and tolerant soybean cultivars
J. Exp. Bot.,
December 1, 2003;
54(393):
2745 - 2756.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Kihara, T. Wada, Y. Suzuki, T. Hara, and H. Koyama
Alteration of Citrate Metabolism in Cluster Roots of White Lupin
Plant Cell Physiol.,
September 15, 2003;
44(9):
901 - 908.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. M. Anoop, U. Basu, M. T. McCammon, L. McAlister-Henn, and G. J. Taylor
Modulation of Citrate Metabolism Alters Aluminum Tolerance in Yeast and Transgenic Canola Overexpressing a Mitochondrial Citrate Synthase
Plant Physiology,
August 1, 2003;
132(4):
2205 - 2217.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Hayes and J. F. Ma
Al-induced efflux of organic acid anions is poorly associated with internal organic acid metabolism in triticale roots
J. Exp. Bot.,
July 1, 2003;
54(388):
1753 - 1759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Kobayashi and H. Koyama
QTL Analysis of Al Tolerance in Recombinant Inbred Lines of Arabidopsis thaliana
Plant Cell Physiol.,
December 15, 2002;
43(12):
1526 - 1533.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Sasaki, B. Ezaki, and H. Matsumoto
A Gene Encoding Multidrug Resistance (MDR)-Like Protein is Induced by Aluminum and Inhibitors of Calcium Flux in Wheat
Plant Cell Physiol.,
February 1, 2002;
43(2):
177 - 185.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Tesfaye, S. J. Temple, D. L. Allan, C. P. Vance, and D. A. Samac
Overexpression of Malate Dehydrogenase in Transgenic Alfalfa Enhances Organic Acid Synthesis and Confers Tolerance to Aluminum
Plant Physiology,
December 1, 2001;
127(4):
1836 - 1844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Ezaki, M. Katsuhara, M. Kawamura, and H. Matsumoto
Different Mechanisms of Four Aluminum (Al)-Resistant Transgenes for Al Toxicity in Arabidopsis
Plant Physiology,
November 1, 2001;
127(3):
918 - 927.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
