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Plant Physiol, April 2001, Vol. 125, pp. 2104-2119

Differential Regulation of a Family of Apyrase Genes from Medicago truncatula1

Jonathan R. Cohn, Taesik Uhm, Senthil Ramu, Yong-Woo Nam, Dong-Jin Kim, R. Varma Penmetsa, Todd C. Wood, Roxanne L. Denny, Nevin D. Young, Douglas R. Cook, and Gary Stacey*

Center for Legume Research, Department of Microbiology, University of Tennessee, Knoxville, Tennessee 37996 (J.R.C., G.S.); Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas (T.U., S.R., Y.-W.N., D.-J.K., R.V.P., D.R.C.); Clemson University Genomics Institute, Clemson University, Clemson, South Carolina (T.C.W.); and Departments of Plant Pathology (R.L.D., N.D.Y.) and Plant Biology (N.D.Y.), University of Minnesota, Minneapolis, Minnesota 55108

Four putative apyrase genes were identified from the model legume Medicago truncatula. Two of the genes identified from M. truncatula (Mtapy1 and Mtapy4) are expressed in roots and are inducible within 3 h after inoculation with Sinorhizobium meliloti. The level of mRNA expression of the other two putative apyrases, Mtapy2 and Mtapy3, was unaffected by rhizobial inoculation. Screening of a bacterial artificial chromosome library of M. truncatula genomic DNA showed that Mtapy1, Mtapy3, and Mtapy4 are present on a single bacterial artificial chromosome clone. This apyrase cluster was mapped to linkage group seven. A syntenic region on soybean linkage group J was found to contain at least two apyrase genes. Screening of nodulation deficient mutants of M. truncatula revealed that two such mutants do not express apyrases to any detectable level. The data suggest a role for apyrases early in the nodulation response before the involvement of root cortical cell division leading to the nodule structure.

1 This work was supported by the U.S. Department of Energy (grant no. DE-FG02-97ER20260 to G.S.) and by the National Science Foundation (grant no. 9872664 to D.R.C. and N.D.Y.).

* Corresponding author; e-mail gstacey{at}utk.edu; fax 865-974-4007.

© 2001 American Society of Plant Physiologists


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