|
Plant Physiol, April 2001, Vol. 125, pp. 2139-2153
Transgenic Manipulation of the Metabolism of Polyamines in Poplar
Cells1
Pratiksha
Bhatnagar,
Bernadette M.
Glasheen,
Suneet K.
Bains,
Stephanie L.
Long,
Rakesh
Minocha,
Christian
Walter, and
Subhash C.
Minocha*
Department of Plant Biology, University of New Hampshire, Durham,
New Hampshire 03824 (P.B., B.M.G., S.K.B., S.C.M.); U.S. Department of
Agriculture Forest Service, Northeastern Experiment Station, P.O. Box
640, Durham, New Hampshire 03824 (S.L.L., R.M.); and New Zealand
Forestry, Private Bag 2030, Rotorua, New Zealand (C.W.)
The metabolism of polyamines (putrescine, spermidine, and spermine)
has become the target of genetic manipulation because of their
significance in plant development and possibly stress tolerance. We
studied the polyamine metabolism in non-transgenic (NT) and transgenic
cells of poplar (Populus nigra × maximowiczii) expressing a mouse Orn decarboxylase
(odc) cDNA. The transgenic cells showed elevated levels
of mouse ODC enzyme activity, severalfold higher amounts of putrescine,
a small increase in spermidine, and a small reduction in spermine as
compared with NT cells. The conversion of labeled ornithine (Orn) into
putrescine was significantly higher in the transgenic than the NT
cells. Whereas exogenously supplied Orn caused an increase in cellular
putrescine in both cell lines, arginine at high concentrations was
inhibitory to putrescine accumulation. The addition of urea and
glutamine had no effect on polyamines in either of the cell lines.
Inhibition of glutamine synthetase by methionine sulfoximine led to a
substantial reduction in putrescine and spermidine in both cell lines.
The results show that: (a) Transgenic expression of a heterologous odc gene can be used to modulate putrescine metabolism
in plant cells, (b) accumulation of putrescine in high amounts does not affect the native arginine decarboxylase activity, (c) Orn biosynthesis occurs primarily from glutamine/glutamate and not from catabolic breakdown of arginine, (d) Orn biosynthesis may become a limiting factor for putrescine production in the odc transgenic
cells, and (e) assimilation of nitrogen into glutamine keeps pace with an increased demand for its use for putrescine production.
1
This work was supported by the University of New
Hampshire Undergraduate Research Opportunity Program, by the U.S.
Department of Agriculture Forest Service, and by the New Zealand Forest
Research Institute (Rotorua). This is New Hampshire Agricultural
Experiment Station contribution no. 2052.
*
Corresponding author; e-mail sminocha{at}christa.unh.edu; fax
603-862-3784.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
T. N. C. Ramya, K. Karmodiya, A. Surolia, and N. Surolia
15-Deoxyspergualin Primarily Targets the Trafficking of Apicoplast Proteins in Plasmodium falciparum
J. Biol. Chem.,
March 2, 2007;
282(9):
6388 - 6397.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. S. Lim, T. R. Chitra, P. Han, E. C. Pua, and H. Yu
Cloning and characterization of Arabidopsis and Brassica juncea flavin-containing amine oxidases
J. Exp. Bot.,
December 1, 2006;
57(15):
4155 - 4169.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Delis, M. Dimou, E. Flemetakis, G. Aivalakis, and P. Katinakis
A root- and hypocotyl-specific gene coding for copper-containing amine oxidase is related to cell expansion in soybean seedlings
J. Exp. Bot.,
January 1, 2006;
57(1):
101 - 111.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Paschalidis and K. A. Roubelakis-Angelakis
Sites and Regulation of Polyamine Catabolism in the Tobacco Plant. Correlations with Cell Division/Expansion, Cell Cycle Progression, and Vascular Development
Plant Physiology,
August 1, 2005;
138(4):
2174 - 2184.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. A. Paschalidis and K. A. Roubelakis-Angelakis
Spatial and Temporal Distribution of Polyamine Levels and Polyamine Anabolism in Different Organs/Tissues of the Tobacco Plant. Correlations with Age, Cell Division/Expansion, and Differentiation
Plant Physiology,
May 1, 2005;
138(1):
142 - 152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-J. Hao, H. Kitashiba, C. Honda, K. Nada, and T. Moriguchi
Expression of arginine decarboxylase and ornithine decarboxylase genes in apple cells and stressed shoots
J. Exp. Bot.,
April 1, 2005;
56(414):
1105 - 1115.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Mayer and A. J. Michael
Polyamine Homeostasis in Transgenic Plants Overexpressing Ornithine Decarboxylase Includes Ornithine Limitation
J. Biochem.,
November 1, 2003;
134(5):
765 - 772.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Bhatnagar, R. Minocha, and S. C. Minocha
Genetic Manipulation of the Metabolism of Polyamines in Poplar Cells. The Regulation of Putrescine Catabolism
Plant Physiology,
April 1, 2002;
128(4):
1455 - 1469.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
