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Plant Physiol, May 2001, Vol. 126, pp. 133-144
Sugar-Binding Activity of Pea Lectin Enhances Heterologous
Infection of Transgenic Alfalfa Plants by Rhizobium
leguminosarum biovar viciae1
Pieternel
van Rhijn,2
Nancy A.
Fujishige,
Pyung Ok
Lim,3 and
Ann M.
Hirsch*
Department of Molecular, Cell, and Developmental Biology (P.v.R.,
N.A.F., P.O.L., A.M.H.) and Molecular Biology Institute (A.M.H.), 405 Hilgard Avenue, University of California, Los Angeles, California
90095-1606
Transgenic alfalfa (Medicago sativa L. cv Regen)
roots carrying genes encoding soybean lectin or pea (Pisum
sativum) seed lectin (PSL) were inoculated with
Bradyrhizobium japonicum or Rhizobium
leguminosarum bv viciae, respectively, and their
responses were compared with those of comparably inoculated control
plants. We found that nodule-like structures formed on alfalfa roots
only when the rhizobial strains produced Nod factor from the
alfalfa-nodulating strain, Sinorhizobium meliloti.
Uninfected nodule-like structures developed on the soybean
lectin-transgenic plant roots at very low inoculum concentrations, but
bona fide infection threads were not detected even when B.
japonicum produced the appropriate S. meliloti
Nod factor. In contrast, the PSL-transgenic plants were not only well
nodulated but also exhibited infection thread formation in response to
R. leguminosarum bv viciae, but only when
the bacteria expressed the complete set of S. meliloti
nod genes. A few nodules from the PSL-transgenic plant roots
were even found to be colonized by R. leguminosarum bv
viciae expressing S. meliloti nod genes, but the plants were yellow and senescent, indicating that nitrogen fixation did not take place. Exopolysaccharide appears to be absolutely required for both nodule development and infection thread formation because neither occurred in PSL-transgenic plant roots following inoculation with an Exo R. leguminosarum
bv viciae strain that produced S.
meliloti Nod factor.
1
This work was supported by the National Research
Competitive Grant Program (grant nos. 93-37305-9144 and
96-35305-3583 to A.M.H.) and by the D. Collen Research Foundation,
K.U. Leuven, Belgium (to P.v.R.).
2
Present address: Laboratorium of Biomolecular Dynamics,
University of Leuven, Celestijnenlaan 200D, 3001 Heverlee, Belgium.
3
Present address: Department of Life Sciences,
Pohang University of Science and Technology, Pohang, Republic of Korea.
*
Corresponding author; e-mail ahirsch{at}ucla.edu; fax
310-206-5413.
© 2001 American Society of Plant Physiologists
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