|
Plant Physiol, May 2001, Vol. 126, pp. 145-155
Simultaneous Down-Regulation of Caffeic/5-Hydroxy Ferulic
Acid-O-Methyltransferase I and Cinnamoyl-Coenzyme A
Reductase in the Progeny from a Cross between Tobacco Lines
Homozygous for Each Transgene. Consequences for Plant Development and
Lignin Synthesis1
Gaelle
Pinçon,
Matthieu
Chabannes,
Catherine
Lapierre,
Brigitte
Pollet,
Katia
Ruel,
Jean-Paul
Joseleau,
Alain M.
Boudet, and
Michel
Legrand*
Institut de Biologie Moléculaire des Plantes du Centre
National de la Recherche Scientifique, Université Louis Pasteur,
67084 Strasbourg cedex, France (G.P., M.L.); Pôle de
Biotechnologies Végétales, Centre National de la Recherche
Scientifique-Université Paul Sabatier, 31326 Castanet-Tolosan,
France (M.C., A.B.); Laboratoire de Chimie Biologique, Institut
National Agronomique, 78850 Thiverval-Grignon, France (C.L., B.P.); and
Centre de Recherche sur les Macromolécules Végétales
du Centre National de la Recherche Scientifique, 38041 Grenoble cedex
9, France (K.R., J.-P.J.)
Inhibition of specific lignin biosynthetic steps by antisense
strategy has previously been shown to alter lignin content and/or structure. In this work, homozygous tobacco (Nicotiana
tabacum) lines transformed with cinnamoyl-coenzyme A
reductase (CCR) or caffeic acid/5-hydroxy ferulic
acid-O-methyltransferase I (COMT I) antisense
sequences have been crossed and enzyme activities, lignin synthesis,
and cell wall structure of the progeny have been analyzed. In single
transformed parents, CCR inhibition did not affect COMT I expression,
whereas marked increases in CCR activity were observed in COMT I
antisense plants, suggesting potential cross talk between some genes of
the pathway. In the progeny, both CCR and COMT I activities were shown
to be markedly decreased due to the simultaneous repression of the two
genes. In these double transformants, the lignin profiles were
dependent on the relative extent of down-regulation of each individual
enzyme. For the siblings issued from a strongly repressed antisense CCR parent, the lignin patterns mimicked the patterns obtained in single
transformants with a reduced CCR activity. In contrast, the specific
lignin profile of COMT I repression could not be detected in double
transformed siblings. By transmission electron microscopy some cell
wall loosening was detected in the antisense CCR parent but not in the
antisense COMT I parent. In double transformants, immunolabeling of
non-condensed guaiacyl-syringyl units was weaker and revealed changes
in epitope distribution that specifically affected vessels. Our results
more widely highlight the impact of culture conditions on phenotypes
and gene expression of transformed plants.
1
This work was supported by the Commission of
European Communities (project nos. Optimization of Lignin in Crop and
Industrial Plants through Genetic Engineering AGRE0021 and Tree
Improvement Based on Lignin Engineering CT95-0424) and by the
Ministère de l'Education Nationale, de la Recherche, et de la
Technologie (grant no. ACC-SV14).
*
Corresponding author; e-mail
michel.legrand{at}ibmp-ulp.u-strasbg.fr; fax
33-0-3-88-61-44-42.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
M. Dafny-Yelin, S.-M. Chung, E. L. Frankman, and T. Tzfira
pSAT RNA Interference Vectors: A Modular Series for Multiple Gene Down-Regulation in Plants
Plant Physiology,
December 1, 2007;
145(4):
1272 - 1281.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-C. Leple, R. Dauwe, K. Morreel, V. Storme, C. Lapierre, B. Pollet, A. Naumann, K.-Y. Kang, H. Kim, K. Ruel, et al.
Downregulation of Cinnamoyl-Coenzyme A Reductase in Poplar: Multiple-Level Phenotyping Reveals Effects on Cell Wall Polymer Metabolism and Structure
PLANT CELL,
November 1, 2007;
19(11):
3669 - 3691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Besseau, L. Hoffmann, P. Geoffroy, C. Lapierre, B. Pollet, and M. Legrand
Flavonoid Accumulation in Arabidopsis Repressed in Lignin Synthesis Affects Auxin Transport and Plant Growth
PLANT CELL,
January 1, 2007;
19(1):
148 - 162.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Zhong, M. J. Pena, G.-K. Zhou, C. J. Nairn, A. Wood-Jones, E. A. Richardson, W. H. Morrison III, A. G. Darvill, W. S. York, and Z.-H. Ye
Arabidopsis Fragile Fiber8, Which Encodes a Putative Glucuronyltransferase, Is Essential for Normal Secondary Wall Synthesis
PLANT CELL,
December 1, 2005;
17(12):
3390 - 3408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Hoffmann, S. Besseau, P. Geoffroy, C. Ritzenthaler, D. Meyer, C. Lapierre, B. Pollet, and M. Legrand
Silencing of Hydroxycinnamoyl-Coenzyme A Shikimate/Quinate Hydroxycinnamoyltransferase Affects Phenylpropanoid Biosynthesis
PLANT CELL,
June 1, 2004;
16(6):
1446 - 1465.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Li, Y. Zhou, X. Cheng, J. Sun, J. M. Marita, J. Ralph, and V. L. Chiang
Combinatorial modification of multiple lignin traits in trees through multigene cotransformation
PNAS,
April 15, 2003;
100(8):
4939 - 4944.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Abbott, A. Barakate, G. Pincon, M. Legrand, C. Lapierre, I. Mila, W. Schuch, and C. Halpin
Simultaneous Suppression of Multiple Genes by Single Transgenes. Down-Regulation of Three Unrelated Lignin Biosynthetic Genes in Tobacco
Plant Physiology,
March 1, 2002;
128(3):
844 - 853.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
