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Plant Physiol, May 2001, Vol. 126, pp. 156-166
Enzymes That Scavenge Reactive Oxygen Species Are Down-Regulated
Prior to Gibberellic Acid-Induced Programmed Cell Death in Barley
Aleurone1
Angelika
Fath,*
Paul C.
Bethke, and
Russell L.
Jones
Department of Plant and Microbial Biology, University of
California, Berkeley, California 94720-3102
Gibberellins (GAs) initiate a series of events that culminate in
programmed cell death, whereas abscisic acid (ABA) prevents this
process. Reactive oxygen species (ROS) are key elements in aleurone
programmed cell death. Incubation of barley (Hordeum vulgare) aleurone layers in H2O2 causes
rapid death of all cells in GA- but not ABA-treated layers. Sensitivity
to H2O2 in GA-treated aleurone cells results
from a decreased ability to metabolize ROS. The amounts and activities
of ROS scavenging enzymes, including catalase (CAT), ascorbate
peroxidase, and superoxide dismutase are strongly down-regulated in
aleurone layers treated with GA. CAT activity, protein, and
Cat2 mRNA decline rapidly following exposure of aleurone
layers to GA. In ABA-treated layers, on the other hand, the amount and
activity of CAT and Cat2 mRNA increases. Incubation in
ABA maintains high amounts of ascorbate peroxidase and superoxide
dismutase, whereas GA brings about a rapid reduction in the amounts of
these enzymes. These data imply that GA-treated cells loose their
ability to scavenge ROS and that this loss ultimately results in
oxidative damage and cell death. ABA-treated cells, on the other hand,
maintain their ability to scavenge ROS and remain viable.
1
This work was supported by the National Science
Foundation (grant no. IBN-9818047) and by Novartis Agricultural
Discovery Institute (to R.L.J.).
*
Corresponding author; e-mail afath{at}nature.berkeley.edu; fax
510-642-4995.
© 2001 American Society of Plant Physiologists
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