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Plant Physiol, May 2001, Vol. 126, pp. 176-187
Sulfur Assimilation in Developing Lupin Cotyledons Could
Contribute Significantly to the Accumulation of Organic Sulfur Reserves
in the Seed
Linda Marie
Tabe* and
Michel
Droux
Commonwealth Scientific and Industrial Research Organization, Plant
Industry, G.P.O. Box 1600, Canberra, Australian Capital Territory 2601, Australia (L.M.T.); and Laboratoire Mixte Centre National de la
Recherche Scientifique-Institut National de la Recherche
Agronomique-Aventis, Unité Mixte de Recherche 1932, Aventis
CropScience, 14-20 Rue Pierre Baizet BP 9163, 69263 Lyon cedex 03, France (M.D.)
It is currently assumed that the assimilation of sulfur into
reduced forms occurs predominantly in the leaves of plants. However, developing seeds have a strong requirement for sulfur amino acids for
storage protein synthesis. We have assessed the capacity of developing
seeds of narrow-leaf lupin (Lupinus angustifolius) for
sulfur assimilation. Cotyledons of developing lupin seeds were able to
transfer the sulfur atom from 35S-labeled sulfate into seed
proteins in vitro, demonstrating the ability of the developing
cotyledons to perform all the steps of sulfur reduction and sulfur
amino acid biosynthesis. Oxidized sulfur constituted approximately 30%
of the sulfur in mature seeds of lupins grown in the field and almost
all of the sulfur detected in phloem exuded from developing pods. The
activities of three enzymes of the sulfur amino acid biosynthetic
pathway were found in developing cotyledons in quantities theoretically
sufficient to account for all of the sulfur amino acids that accumulate
in the protein of mature lupin seeds. We conclude that sulfur
assimilation by developing cotyledons is likely to be an important
source of sulfur amino acids for the synthesis of storage proteins
during lupin seed maturation.
*
Corresponding author; e-mail L.Tabe{at}pi.csiro.au; fax
61-2-62465000.
© 2001 American Society of Plant Physiologists
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