Plant Physiol. Illumina
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow PPT slides of all figures
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (41)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Orfila, C.
Right arrow Articles by Knox, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Orfila, C.
Right arrow Articles by Knox, J. P.
Agricola
Right arrow Articles by Orfila, C.
Right arrow Articles by Knox, J. P.

Plant Physiol, May 2001, Vol. 126, pp. 210-221

Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1right-arrow 5)-alpha -L-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato1

Caroline Orfila, Graham B. Seymour, William G.T. Willats, I. Max Huxham, Michael C. Jarvis, Colin J. Dover, Andrew J. Thompson, and J. Paul Knox*

Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (C.O., W.G.T.W., J.P.K.); Horticulture Research International, Wellesbourne, Warwick CV35 9EF, United Kingdom (G.B.S., A.J.T.); Cell and Molecular Biology Division, Institute of Biomedical and Life Sciences (I.M.H.) and Department of Chemistry (M.C.J.), University of Glasgow, Glasgow G12 8QQ, United Kingdom; and Horticulture Research International, East Malling, West Malling, Kent ME19 6BJ, United Kingdom (C.J.D.)

Cnr (colorless non-ripening) is a pleiotropic tomato (Lycopersicon esculentum) fruit ripening mutant with altered tissue properties including weaker cell-to-cell contacts in the pericarp (A.J. Thompson, M. Tor, C.S. Barry, J. Vrebalov, C. Orfila, M.C. Jarvis, J.J. Giovannoni, D. Grierson, G.B. Seymour [1999] Plant Physiol 120: 383-390). Whereas the genetic basis of the Cnr mutation is being identified by molecular analyses, here we report the identification of cell biological factors underlying the Cnr texture phenotype. In comparison with wild type, ripe-stage Cnr fruits have stronger, non-swollen cell walls (CW) throughout the pericarp and extensive intercellular space in the inner pericarp. Using electron energy loss spectroscopy imaging of calcium-binding capacity and anti-homogalacturonan (HG) antibody probes (PAM1 and JIM5) we demonstrate that maturation processes involving middle lamella HG are altered in Cnr fruit, resulting in the absence or a low level of HG-/calcium-based cell adhesion. We also demonstrate that the deposition of (1right-arrow5)-alpha -L-arabinan is disrupted in Cnr pericarp CW and that this disruption occurs prior to fruit ripening. The relationship between the disruption of (1right-arrow5)-alpha -L-arabinan deposition in pericarp CW and the Cnr phenotype is discussed.

1 This work was supported by the United Kingdom Biotechnology and Biological Sciences Research Council (G.B.S., A.J.T.), including a Co-operative Award in Science and Engineering studentship with Horticulture Research International (to C.O.) and a grant (no. 17/DC09694 to I.M.H.).

* Corresponding author; e-mail j.p.knox{at}leeds.ac.uk; fax 44-113-2333144.

© 2001 American Society of Plant Physiologists


This article has been cited by other articles:


Home page
 Mol PlantHome page
J. J. Ordaz-Ortiz, S. E. Marcus, and J. Paul Knox
Cell Wall Microstructure Analysis Implicates Hemicellulose Polysaccharides in Cell Adhesion in Tomato Fruit Pericarp Parenchyma
Mol Plant, September 1, 2009; 2(5): 910 - 921.
[Abstract] [Full Text] [PDF]

Home page
 Mol PlantHome page
L. D. Gomez, C. G. Steele-King, L. Jones, J. M. Foster, S. Vuttipongchaikij, and S. J. McQueen-Mason
Arabinan Metabolism during Seed Development and Germination in Arabidopsis
Mol Plant, September 1, 2009; 2(5): 966 - 976.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. A. Arsovski, T. M. Popma, G. W. Haughn, N. C. Carpita, M. C. McCann, and T. L. Western
AtBXL1 Encodes a Bifunctional {beta}-D-Xylosidase/{alpha}-L-Arabinofuranosidase Required for Pectic Arabinan Modification in Arabidopsis Mucilage Secretory Cells
Plant Physiology, July 1, 2009; 150(3): 1219 - 1234.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. J. Bernal, C.-M. Yoo, M. Mutwil, J. K. Jensen, G. Hou, C. Blaukopf, I. Sorensen, E. B. Blancaflor, H. V. Scheller, and W. G.T. Willats
Functional Analysis of the Cellulose Synthase-Like Genes CSLD1, CSLD2, and CSLD4 in Tip-Growing Arabidopsis Cells
Plant Physiology, November 1, 2008; 148(3): 1238 - 1253.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Gonzalez-Aguero, L. Pavez, F. Ibanez, I. Pacheco, R. Campos-Vargas, L. A. Meisel, A. Orellana, J. Retamales, H. Silva, M. Gonzalez, et al.
Identification of woolliness response genes in peach fruit after post-harvest treatments
J. Exp. Bot., May 3, 2008; (2008) ern069v1.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
F. Guillon, S. Philippe, B. Bouchet, M.-F. Devaux, P. Frasse, B. Jones, M. Bouzayen, and M. Lahaye
Down-regulation of an Auxin Response Factor in the tomato induces modification of fine pectin structure and tissue architecture
J. Exp. Bot., February 10, 2008; (2008) erm323v1.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Siedlecka, S. Wiklund, M.-A. Peronne, F. Micheli, J. Lesniewska, I. Sethson, U. Edlund, L. Richard, B. Sundberg, and E. J. Mellerowicz
Pectin Methyl Esterase Inhibits Intrusive and Symplastic Cell Growth in Developing Wood Cells of Populus
Plant Physiology, February 1, 2008; 146(2): 554 - 565.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. D. Phan, W. Bo, G. West, G. W. Lycett, and G. A. Tucker
Silencing of the Major Salt-Dependent Isoform of Pectinesterase in Tomato Alters Fruit Softening
Plant Physiology, August 1, 2007; 144(4): 1960 - 1967.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T. Konishi, H. Ono, M. Ohnishi-Kameyama, S. Kaneko, and T. Ishii
Identification of a Mung Bean Arabinofuranosyltransferase That Transfers Arabinofuranosyl Residues onto (1, 5)-Linked {alpha}-L-Arabino-Oligosaccharides
Plant Physiology, July 1, 2006; 141(3): 1098 - 1105.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. P. Moore, E. Nguema-Ona, L. Chevalier, G. G. Lindsey, W. F. Brandt, P. Lerouge, J. M. Farrant, and A. Driouich
Response of the Leaf Cell Wall to Desiccation in the Resurrection Plant Myrothamnus flabellifolius
Plant Physiology, June 1, 2006; 141(2): 651 - 662.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Harholt, J. K. Jensen, S. O. Sorensen, C. Orfila, M. Pauly, and H. V. Scheller
ARABINAN DEFICIENT 1 Is a Putative Arabinosyltransferase Involved in Biosynthesis of Pectic Arabinan in Arabidopsis
Plant Physiology, January 1, 2006; 140(1): 49 - 58.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Leboeuf, F. Guillon, S. Thoiron, and M. Lahaye
Biochemical and immunohistochemical analysis of pectic polysaccharides in the cell walls of Arabidopsis mutant QUASIMODO 1 suspension-cultured cells: implications for cell adhesion
J. Exp. Bot., December 1, 2005; 56(422): 3171 - 3182.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Tateishi, H. Mori, J. Watari, K. Nagashima, S. Yamaki, and H. Inoue
Isolation, Characterization, and Cloning of {alpha}-L-Arabinofuranosidase Expressed during Fruit Ripening of Japanese Pear
Plant Physiology, July 1, 2005; 138(3): 1653 - 1664.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. M. Eriksson, A. Bovy, K. Manning, L. Harrison, J. Andrews, J. De Silva, G. A. Tucker, and G. B. Seymour
Effect of the Colorless non-ripening Mutation on Cell Wall Biochemistry and Gene Expression during Tomato Fruit Development and Ripening
Plant Physiology, December 1, 2004; 136(4): 4184 - 4197.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
E. Leboeuf, S. Thoiron, and M. Lahaye
Physico-chemical characteristics of cell walls from Arabidopsis thaliana microcalli showing different adhesion strengths
J. Exp. Bot., September 1, 2004; 55(405): 2087 - 2097.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. A. Brummell, V. Dal Cin, S. Lurie, C. H. Crisosto, and J. M. Labavitch
Cell wall metabolism during the development of chilling injury in cold-stored peach fruit: association of mealiness with arrested disassembly of cell wall pectins
J. Exp. Bot., September 1, 2004; 55(405): 2041 - 2052.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. Capodicasa, D. Vairo, O. Zabotina, L. McCartney, C. Caprari, B. Mattei, C. Manfredini, B. Aracri, J. Benen, J. P. Knox, et al.
Targeted Modification of Homogalacturonan by Transgenic Expression of a Fungal Polygalacturonase Alters Plant Growth
Plant Physiology, July 1, 2004; 135(3): 1294 - 1304.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. J. Pena and N. C. Carpita
Loss of Highly Branched Arabinans and Debranching of Rhamnogalacturonan I Accompany Loss of Firm Texture and Cell Separation during Prolonged Storage of Apple
Plant Physiology, July 1, 2004; 135(3): 1305 - 1313.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
J. J. Giovannoni
Genetic Regulation of Fruit Development and Ripening
PLANT CELL, June 1, 2004; 16(suppl_1): S170 - S180.
[Full Text] [PDF]

Home page
 J Exp BotHome page
L. M. Fulton and C. S. Cobbett
Two {alpha}-L-arabinofuranosidase genes in Arabidopsis thaliana are differentially expressed during vegetative growth and flower development
J. Exp. Bot., November 1, 2003; 54(392): 2467 - 2477.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Freshour, C. P. Bonin, W.-D. Reiter, P. Albersheim, A. G. Darvill, and M. G. Hahn
Distribution of Fucose-Containing Xyloglucans in Cell Walls of the mur1 Mutant of Arabidopsis
Plant Physiology, April 1, 2003; 131(4): 1602 - 1612.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. M. Lord and J.-C. Mollet
Plant cell adhesion: A bioassay facilitates discovery of the first pectin biosynthetic gene
PNAS, December 10, 2002; 99(25): 15843 - 15845.
[Full Text] [PDF]

Home page
 J Exp BotHome page
G. B. Seymour, K. Manning, E. M. Eriksson, A. H. Popovich, and G. J. King
Genetic identification and genomic organization of factors affecting fruit texture
J. Exp. Bot., October 1, 2002; 53(377): 2065 - 2071.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
S. Bouton, E. Leboeuf, G. Mouille, M.-T. Leydecker, J. Talbotec, F. Granier, M. Lahaye, H. Hofte, and H.-N. Truong
QUASIMODO1 Encodes a Putative Membrane-Bound Glycosyltransferase Required for Normal Pectin Synthesis and Cell Adhesion in Arabidopsis
PLANT CELL, October 1, 2002; 14(10): 2577 - 2590.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. G. Atkinson, R. Schroder, I. C. Hallett, D. Cohen, and E. A. MacRae
Overexpression of Polygalacturonase in Transgenic Apple Trees Leads to a Range of Novel Phenotypes Involving Changes in Cell Adhesion
Plant Physiology, May 1, 2002; 129(1): 122 - 133.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 2001 by the American Society of Plant Biologists