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Plant Physiol, May 2001, Vol. 126, pp. 210-221

Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1right-arrow 5)-alpha -L-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato1

Caroline Orfila, Graham B. Seymour, William G.T. Willats, I. Max Huxham, Michael C. Jarvis, Colin J. Dover, Andrew J. Thompson, and J. Paul Knox*

Centre for Plant Sciences, University of Leeds, Leeds LS2 9JT, United Kingdom (C.O., W.G.T.W., J.P.K.); Horticulture Research International, Wellesbourne, Warwick CV35 9EF, United Kingdom (G.B.S., A.J.T.); Cell and Molecular Biology Division, Institute of Biomedical and Life Sciences (I.M.H.) and Department of Chemistry (M.C.J.), University of Glasgow, Glasgow G12 8QQ, United Kingdom; and Horticulture Research International, East Malling, West Malling, Kent ME19 6BJ, United Kingdom (C.J.D.)

Cnr (colorless non-ripening) is a pleiotropic tomato (Lycopersicon esculentum) fruit ripening mutant with altered tissue properties including weaker cell-to-cell contacts in the pericarp (A.J. Thompson, M. Tor, C.S. Barry, J. Vrebalov, C. Orfila, M.C. Jarvis, J.J. Giovannoni, D. Grierson, G.B. Seymour [1999] Plant Physiol 120: 383-390). Whereas the genetic basis of the Cnr mutation is being identified by molecular analyses, here we report the identification of cell biological factors underlying the Cnr texture phenotype. In comparison with wild type, ripe-stage Cnr fruits have stronger, non-swollen cell walls (CW) throughout the pericarp and extensive intercellular space in the inner pericarp. Using electron energy loss spectroscopy imaging of calcium-binding capacity and anti-homogalacturonan (HG) antibody probes (PAM1 and JIM5) we demonstrate that maturation processes involving middle lamella HG are altered in Cnr fruit, resulting in the absence or a low level of HG-/calcium-based cell adhesion. We also demonstrate that the deposition of (1right-arrow5)-alpha -L-arabinan is disrupted in Cnr pericarp CW and that this disruption occurs prior to fruit ripening. The relationship between the disruption of (1right-arrow5)-alpha -L-arabinan deposition in pericarp CW and the Cnr phenotype is discussed.

1 This work was supported by the United Kingdom Biotechnology and Biological Sciences Research Council (G.B.S., A.J.T.), including a Co-operative Award in Science and Engineering studentship with Horticulture Research International (to C.O.) and a grant (no. 17/DC09694 to I.M.H.).

* Corresponding author; e-mail j.p.knox{at}leeds.ac.uk; fax 44-113-2333144.

© 2001 American Society of Plant Physiologists


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