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Plant Physiol, May 2001, Vol. 126, pp. 299-306
Phytochelatins Are Involved in Differential Arsenate Tolerance in
Holcus lanatus1
Jeanette
Hartley-Whitaker,*
Gillian
Ainsworth,
Riet
Vooijs,
Wilma
Ten
Bookum,
Henk
Schat, and
Andrew A.
Meharg
Centre for Ecology and Hydrology, Merlewood, Grange-over-Sands,
Cumbria, LA11 6JU, United Kingdom (J.H.-W., G.A.); Department of
Ecology and Ecotoxicology of Plants, Vrije Universiteit, De Boelelaan
1087, 1081 HV Amsterdam, The Netherlands (R.V., W.T.B., H.S.); and
Department of Plant and Soil Science, University of Aberdeen, Aberdeen,
AB24 3UU, Scotland (A.A.M.)
Arsenate tolerance is conferred by suppression of the high-affinity
phosphate/arsenate uptake system, which greatly reduces arsenate influx
in a number of higher plant species. Despite this suppressed uptake,
arsenate-tolerant plants can still accumulate high levels of As over
their lifetime, suggesting that constitutive detoxification mechanisms
may be required. Phytochelatins are thiol-rich peptides, whose
production is induced by a range of metals and metalloids including
arsenate. This study provides evidence for the role of phytochelatins
in the detoxification of arsenate in arsenate-tolerant Holcus
lanatus. Elevated levels of phytochelatin were measured in
plants with a range of tolerance to arsenate at equivalent levels of
arsenate stress, measured as inhibition of root growth. The results
suggest that arsenate tolerance in H. lanatus requires
both adaptive suppression of the high-affinity phosphate uptake system
and constitutive phytochelatin production.
1
This work was supported by the Natural
Environment Research Council, U.K. and by COST Action 837 Short Term
Scientific Mission (to J.H.W.). .
*
Corresponding author; e-mail jhart{at}ceh.ac.uk; fax 44-15395-34705
© 2001 American Society of Plant Physiologists
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