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Plant Physiol, May 2001, Vol. 126, pp. 363-375
Genes That Are Uniquely Stress Regulated in Salt Overly Sensitive
(sos) Mutants1
Zhizhong
Gong,2
Hisashi
Koiwa,
Mary Ann
Cushman,
Anamika
Ray,
Davi
Bufford,
Shin
Kore-eda,
Tracie K.
Matsumoto,
Jianhua
Zhu,
John C.
Cushman,
Ray A.
Bressan, and
Paul M.
Hasegawa*
Center for Plant Environmental Stress Physiology, 1165 Horticulture Building, Purdue University, West Lafayette, Indiana
47907-1165 (Z.G., H.K., T.K.M., J.Z., R.A.B., P.M.H.); Department of
Biochemistry MS200, University of Nevada, Reno, Nevada
89557-0014 (M.A.C., S.K., J.C.C.); and Department of Biochemistry and
Molecular Biology, Oklahoma State University, Stillwater, Oklahoma
74078 (A.R., D.B.)
Repetitive rounds of differential subtraction screening, followed
by nucleotide sequence determination and northern-blot analysis, identified 84 salt-regulated (160 mM NaCl for 4 h)
genes in Arabidopsis wild-type (Col-0 gl1) seedlings.
Probes corresponding to these 84 genes and ACP1,
RD22BP1, MYB2, STZ, and
PAL were included in an analysis of salt responsive gene
expression profiles in gl1 and the salt-hypersensitive
mutant sos3. Six of 89 genes were expressed
differentially in wild-type and sos3 seedlings;
steady-state mRNA abundance of five genes
(AD06C08/unknown, AD05E05/vegetative storage protein 2 [VSP2],
AD05B11/S-adenosyl-L-Met:salicylic acid carboxyl methyltransferase [SAMT],
AD03D05/cold regulated 6.6/inducible2 [COR6.6/KIN2], and salt tolerance zinc finger
[STZ]) was induced and the abundance of one gene
(AD05C10/circadian rhythm-RNA binding1 [CCR1]) was reduced in wild-type plants after salt
treatment. The expression of CCR1, SAMT,
COR6.6/KIN2, and STZ was higher in
sos3 than in wild type, and VSP2 and
AD06C08/unknown was lower in the mutant. Salt-induced
expression of VSP2 in sos1 was similar to
wild type, and AD06C08/unknown, CCR1,
SAMT, COR6.6/KIN2, and STZ
were similar to sos3. VSP2 is regulated presumably by
SOS2/3 independent of SOS1, whereas the
expression of the others is SOS1 dependent.
AD06C08/unknown and VSP2 are postulated
to be effectors of salt tolerance whereas CCR1,
SAMT, COR6.6/KIN2, and STZ
are determinants that must be negatively regulated during salt
adaptation. The pivotal function of the SOS signal pathway to mediate
ion homeostasis and salt tolerance implicates
AD06C08/unknown, VSP2, SAMT, 6.6/KIN2, STZ, and
CCR1 as determinates that are involved in salt adaptation.
1
This work was supported by a National Science
Foundation Plant Genome award (no. DBI-9813360). This is Purdue
University Agricultural Experiment Station paper no. 16427.
2
Present address: Department of Plant Sciences,
University of Arizona, Tucson, AZ 85721.
*
Corresponding author; e-mail paul.m.hasegawa.1{at}purdue.edu;
fax 765-494-0391.
© 2001 American Society of Plant Physiologists
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