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Plant Physiol, May 2001, Vol. 126, pp. 376-387
Regulation of Alternative Oxidase Activity in Six Wild
Monocotyledonous Species. An in Vivo Study at the Whole Root
Level1
Frank F.
Millenaar,*
Miquel A.
Gonzàlez-Meler,23
Fabio
Fiorani,3
Rob
Welschen,
Miquel
Ribas-Carbo,4
James N.
Siedow,
Anneke M.
Wagner, and
Hans
Lambers5
Plant Ecophysiology, Utrecht University, Sorbonnelaan 16, 3584 CA
Utrecht, The Netherlands (F.F.M., F.F., R.W., H.L.); Botany
Department-Developmental, Cell, and Molecular Biology Group,
Duke University, Durham, North Carolina 27708 (M.A.G.-M., M.R.-C.,
J.N.S.); Department of Molecular Cell Physiology, Vrije Universiteit,
Amsterdam, The Netherlands (A.M.W.); and Plant Sciences, Faculty of
Agriculture, The University of Western Australia, Nedlands WA 6907, Australia (H.L.)
The activity of the alternative pathway is affected by a number of
factors, including the level and reduction state of the alternative
oxidase (AOX) protein, and the reduction state of the ubiquinone pool.
To investigate the significance of these factors for the rate of
alternative respiration in vivo, we studied root respiration of six
wild monocotyledonous grass species that were grown under identical
controlled conditions. The activity of the alternative pathway was
determined using the oxygen isotope fractionation technique. In all
species, the AOX protein was invariably in its reduced (high activity)
state. There was no correlation between AOX activity and AOX protein
concentration, ubiquinone (total, reduced, or oxidized) concentration,
or the reduction state of the ubiquinone pool. However, when some of
these factors are combined in a linear regression model, a good fit to
AOX activity is obtained. The function of the AOX is still not fully
understood. It is interesting that we found a positive correlation
between the activity of the alternative pathway and relative growth
rate; a possible explanation for this correlation is discussed.
Inhibition of the AOX (with salicylhydroxamic acid) decreases
respiration rates less than the activity present before inhibition
(i.e. measured with the 18O-fractionation technique).
1
This work was supported in part by the U.S.
Department of Agriculture National Research Initiative (grant no. CPG
94-37306-0352 to J.N.S.), by the National Science Foundation Division
of Environmental Biology (grant no. DEB-94-15541 to the Duke
University Phytotron), and by the Netherlands Organization for the
Advancement of Science (grant no. SIR 14-2309).
2
These authors contributed equally to the paper.
3
Present address: Department of Biological Sciences,
University of Illinois, 845 West Taylor Street, Chicago, IL 60607.
4
Present address: Department of Plant Biology, Carnegie
Institution of Washington, 260 Panama Street, Stanford, CA 94305.
5
Present address: 35 Stirling Highway, Crawley, Western
Australia 6009, Australia.
*
Corresponding author; e-mail F.F.Millenaar{at}bio.uu.nl; fax
31-302518366.
© 2001 American Society of Plant Physiologists
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