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Plant Physiol, May 2001, Vol. 126, pp. 388-396
A New Approach to Measure Gross CO2 Fluxes in Leaves.
Gross CO2 Assimilation, Photorespiration, and Mitochondrial
Respiration in the Light in Tomato under Drought Stress
Silke
Haupt-Herting,
Klaus
Klug, and
Heinrich P.
Fock*
Fachbereich Biologie der Universität, Postfach 3049, D-67653
Kaiserslautern, Germany
We developed a new method using 13CO2 and
mass spectrometry to elucidate the role of photorespiration as an
alternative electron dissipating pathway under drought stress. This was
achieved by experimentally distinguishing between the CO2
fluxes into and out of the leaf. The method allows us to determine the
rates of gross CO2 assimilation and gross CO2
evolution in addition to net CO2 uptake by attached leaves
during steady-state photosynthesis. Furthermore, a comparison between
measurements under photorespiratory and non-photorespiratory conditions
may give information about the contribution of photorespiration and
mitochondrial respiration to the rate of gross CO2
evolution at photosynthetic steady state. In tomato
(Lycopersicon esculentum Mill. cv Moneymaker) leaves, drought stress decreases the rates of net and gross CO2
uptake as well as CO2 release from photorespiration and
mitochondrial respiration in the light. However, the ratio of
photorespiratory CO2 evolution to gross CO2
assimilation rises with water deficit. Also the contribution of
re-assimilation of (photo) respiratory CO2 to gross
CO2 assimilation increases under drought.
*
Corresponding author; e-mail fock{at}rhrk.uni-kl.de; fax
49-631-205-2600.
© 2001 American Society of Plant Physiologists
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