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Plant Physiol, May 2001, Vol. 126, pp. 421-433
The Light Sensitivity of ATP Synthase Mutants of
Chlamydomonas reinhardtii1
Wojciech
Majeran,
Jacqueline
Olive,
Dominique
Drapier,
Olivier
Vallon, and
Francis-André
Wollman*
Unité Propre de Recherche-Centre National de la Recherche
Scientifique 1261, Institut de Biologie Physico-Chimique, 13 rue Pierre
et Marie Curie, 75005 Paris, France (W.M., D.D., O.V., F.-A.W.);
and Institut Jacques Monod, 2 Place Jussieu, 75251 Paris cedex 05, France (J.O.)
Chlamydomonas reinhardtii mutants defective in the
chloroplast ATP synthase are highly sensitive to light. The ac46 mutant is affected in the MDH1 gene, required for production or
stability of the monocistronic atpH mRNA encoding
CFO-III. In this and other ATP synthase mutants, we
show that short-term exposure to moderate light intensities a few
minutesinduces an inhibition of electron transfer after the primary
quinone acceptor of photosystem II (PSII), whereas longer
exposureseveral hoursleads to a progressive loss of PSII cores. An
extensive swelling of thylakoids accompanies the initial inhibition of
electron flow. Thylakoids deflate as PSII cores are lost. The slow
process of PSII degradation involves the participation of ClpP, a
chloroplast-encoded peptidase that is part of a major stromal protease
Clp. In the light of the above findings, we discuss the
photosensitivity of ATP synthase mutants with respect to the regular
photoinhibition process that affects photosynthetic competent strains
at much higher light intensities.
1
This work was supported by the Centre National
de la Recherche Scientifique (Unité Propre de Recherche 1261) and
by a fellowship from the Ministère de l'Education Nationale (to
W.M.).
*
Corresponding author; e-mail wollman{at}ibpc.fr; fax
33-1-58-41-5012.
© 2001 American Society of Plant Physiologists
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