|
Plant Physiol, May 2001, Vol. 126, pp. 47-68
The Arabidopsis Cell Plate-Associated Dynamin-Like Protein,
ADL1Ap, Is Required for Multiple Stages of Plant Growth and
Development1
Byung-Ho
Kang,
James S.
Busse,
Carrie
Dickey,
David M.
Rancour, and
Sebastian Y.
Bednarek*
Department of Biochemistry, University of Wisconsin, 433 Babcock
Drive, Madison, Wisconsin 53706
Dynamin and dynamin-like proteins are GTP-binding proteins involved
in vesicle trafficking. In soybean, a 68-kD dynamin-like protein called
phragmoplastin has been shown to be associated with the cell plate in
dividing cells (Gu and Verma, 1996). Five ADL1 genes
encoding dynamin-like proteins related to phragmoplastin have been
identified in the completed Arabidopsis genome. Here we report that
ADL1Ap is associated with punctate subcellular structures and with the
cell plate in dividing cells. To assess the function of ADL1Ap we
utilized a reverse genetic approach to isolate three separate
Arabidopsis mutant lines containing T-DNA insertions in
ADL1A. Homozygous adl1A seeds were
shriveled and mutant seedlings arrested soon after germination,
producing only two leaf primordia and severely stunted roots.
Immunoblotting revealed that ADL1Ap expression was not detectable in
the mutants. Despite the loss of ADL1Ap, the mutants did not display
any defects in cytokinesis, and growth of the mutant seedlings could be
rescued in tissue culture by the addition of sucrose. Although these
sucrose-rescued plants displayed normal vegetative growth and flowered,
they set very few seeds. Thus, ADL1Ap is critical for several stages of plant development, including embryogenesis, seedling development, and
reproduction. We discuss the putative role of ADL1Ap in vesicular trafficking, cytokinesis, and other aspects of plant growth.
1
This research was supported by the U.S.
Department of Agriculture-Plant Growth and Development Project (grant
no. 98-35304-6671), by the U.S. Department of Agriculture Cooperative
State Research Education and Extension Service Project (grant no.
WIS04026), and by a Steenbock Career Development award (to
S.Y.B.).
*
Corresponding author; e-mail bednarek{at}biochem.wisc.edu; fax
608-262-3453.
© 2001 American Society of Plant Physiologists
This article has been cited by other articles:
|
|
|
|
 
X.-Y. Chen, L. Liu, E. Lee, X. Han, Y. Rim, H. Chu, S.-W. Kim, F. Sack, and J.-Y. Kim
The Arabidopsis Callose Synthase Gene GSL8 Is Required for Cytokinesis and Cell Patterning
Plant Physiology,
May 1, 2009;
150(1):
105 - 113.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Q. Phan, S.-J. Kim, and D. C. Bassham
Overexpression of Arabidopsis Sorting Nexin AtSNX2b Inhibits Endocytic Trafficking to the Vacuole
Mol Plant,
November 1, 2008;
1(6):
961 - 976.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Park, D. M. Rancour, and S. Y. Bednarek
In Planta Analysis of the Cell Cycle-Dependent Localization of AtCDC48A and Its Critical Roles in Cell Division, Expansion, and Differentiation
Plant Physiology,
September 1, 2008;
148(1):
246 - 258.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Konopka and S. Y. Bednarek
Comparison of the Dynamics and Functional Redundancy of the Arabidopsis Dynamin-Related Isoforms DRP1A and DRP1C during Plant Development
Plant Physiology,
August 1, 2008;
147(4):
1590 - 1602.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Konopka, S. K. Backues, and S. Y. Bednarek
Dynamics of Arabidopsis Dynamin-Related Protein 1C and a Clathrin Light Chain at the Plasma Membrane
PLANT CELL,
May 1, 2008;
20(5):
1363 - 1380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Collings, L. K. Gebbie, P. A. Howles, U. A. Hurley, R. J. Birch, A. H. Cork, C. H. Hocart, T. Arioli, and R. E. Williamson
Arabidopsis dynamin-like protein DRP1A: a null mutant with widespread defects in endocytosis, cellulose synthesis, cytokinesis, and cell expansion
J. Exp. Bot.,
February 5, 2008;
(2008)
erm324v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. M. Stupar, P. B. Bhaskar, B. S. Yandell, W. A. Rensink, A. L. Hart, S. Ouyang, R. E. Veilleux, J. S. Busse, R. J. Erhardt, C. R. Buell, et al.
Phenotypic and Transcriptomic Changes Associated With Potato Autopolyploidization
Genetics,
August 1, 2007;
176(4):
2055 - 2067.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Van Damme, S. Coutuer, R. De Rycke, F.-Y. Bouget, D. Inze, and D. Geelen
Somatic Cytokinesis and Pollen Maturation in Arabidopsis Depend on TPLATE, Which Has Domains Similar to Coat Proteins
PLANT CELL,
December 1, 2006;
18(12):
3502 - 3518.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sawa, K. Koizumi, S. Naramoto, T. Demura, T. Ueda, A. Nakano, and H. Fukuda
DRP1A Is Responsible for Vascular Continuity Synergistically Working with VAN3 in Arabidopsis
Plant Physiology,
June 1, 2005;
138(2):
819 - 826.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Muangprom, S. G. Thomas, T.-p. Sun, and T. C. Osborn
A Novel Dwarfing Mutation in a Green Revolution Gene from Brassica rapa
Plant Physiology,
March 1, 2005;
137(3):
931 - 938.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Rancour, S. Park, S. D. Knight, and S. Y. Bednarek
Plant UBX Domain-containing Protein 1, PUX1, Regulates the Oligomeric Structure and Activity of Arabidopsis CDC48
J. Biol. Chem.,
December 24, 2004;
279(52):
54264 - 54274.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. K. Peterman, Y. M. Ohol, L. J. McReynolds, and E. J. Luna
Patellin1, a Novel Sec14-Like Protein, Localizes to the Cell Plate and Binds Phosphoinositides
Plant Physiology,
October 1, 2004;
136(2):
3080 - 3094.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Edlund, R. Swanson, and D. Preuss
Pollen and Stigma Structure and Function: The Role of Diversity in Pollination
PLANT CELL,
June 1, 2004;
16(suppl_1):
S84 - S97.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Segui-Simarro, J. R. Austin II, E. A. White, and L. A. Staehelin
Electron Tomographic Analysis of Somatic Cell Plate Formation in Meristematic Cells of Arabidopsis Preserved by High-Pressure Freezing
PLANT CELL,
April 1, 2004;
16(4):
836 - 856.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-i. Arimura, G. P. Aida, M. Fujimoto, M. Nakazono, and N. Tsutsumi
Arabidopsis Dynamin-Like Protein 2a (ADL2a), Like ADL2b, is Involved in Plant Mitochondrial Division
Plant Cell Physiol.,
February 15, 2004;
45(2):
236 - 242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. B. Jin, H. Bae, S. J. Kim, Y. H. Jin, C.-H. Goh, D. H. Kim, Y. J. Lee, Y. C. Tse, L. Jiang, and I. Hwang
The Arabidopsis Dynamin-Like Proteins ADL1C and ADL1E Play a Critical Role in Mitochondrial Morphogenesis
PLANT CELL,
October 1, 2003;
15(10):
2357 - 2369.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. G. Falbel, L. M. Koch, J. A. Nadeau, J. M. Segui-Simarro, F. D. Sack, and S. Y. Bednarek
SCD1 is required for cell cytokinesis and polarized cell expansion in Arabidopsis thaliana
Development,
September 1, 2003;
130(17):
4011 - 4024.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-H. Kang, J. S. Busse, and S. Y. Bednarek
Members of the Arabidopsis Dynamin-Like Gene Family, ADL1, Are Essential for Plant Cytokinesis and Polarized Cell Growth
PLANT CELL,
April 1, 2003;
15(4):
899 - 913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. A. Eckardt
Dynamic Trio: FtsZ, Plastid-Dividing, and Dynamin Rings Control Chloroplast Division
PLANT CELL,
March 1, 2003;
15(3):
577 - 579.
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Rojo, J. Zouhar, V. Kovaleva, S. Hong, and N. V. Raikhel
The AtC-VPS Protein Complex Is Localized to the Tonoplast and the Prevacuolar Compartment in Arabidopsis
Mol. Biol. Cell,
February 1, 2003;
14(2):
361 - 369.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Rancour, C. E. Dickey, S. Park, and S. Y. Bednarek
Characterization of AtCDC48. Evidence for Multiple Membrane Fusion Mechanisms at the Plane of Cell Division in Plants
Plant Physiology,
November 1, 2002;
130(3):
1241 - 1253.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Zheng, S. Y. Bednarek, A. A. Sanderfoot, J. Alonso, J. R. Ecker, and N. V. Raikhel
NPSN11 Is a Cell Plate-Associated SNARE Protein That Interacts with the Syntaxin KNOLLE
Plant Physiology,
June 1, 2002;
129(2):
530 - 539.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. K. Muday and A. S. Murphy
An Emerging Model of Auxin Transport Regulation
PLANT CELL,
February 1, 2002;
14(2):
293 - 299.
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-W. Kim, D.-S. Park, S.-C. Park, S. H. Kim, G.-W. Cheong, and I. Hwang
Arabidopsis Dynamin-Like 2 That Binds Specifically to Phosphatidylinositol 4-Phosphate Assembles into a High-Molecular Weight Complex in Vivo and in Vitro
Plant Physiology,
November 1, 2001;
127(3):
1243 - 1255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Otegui, D. N. Mastronarde, B.-H. Kang, S. Y. Bednarek, and L. A. Staehelin
Three-Dimensional Analysis of Syncytial-Type Cell Plates during Endosperm Cellularization Visualized by High Resolution Electron Tomography
PLANT CELL,
September 1, 2001;
13(9):
2033 - 2051.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. S. Murphy, K. R. Hoogner, W. A. Peer, and L. Taiz
Identification, Purification, and Molecular Cloning of N-1-Naphthylphthalmic Acid-Binding Plasma Membrane-Associated Aminopeptidases from Arabidopsis
Plant Physiology,
March 1, 2002;
128(3):
935 - 950.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
