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Plant Physiol, May 2001, Vol. 126, pp. 69-77
Heat Stress Response in Pea Involves Interaction of Mitochondrial
Nucleoside Diphosphate Kinase with a Novel 86-Kilodalton
Protein1
Martha L.
Escobar Galvis,
Salla
Marttila,
Gunilla
Håkansson,
Jens
Forsberg, and
Carina
Knorpp2*
Plant Biochemistry, Lund University, Box 117, SE-221 00 Lund,
Sweden (M.L.E.G., G.H., J.F., C.K.); and Department of Crop Science,
Swedish University of Agricultural Sciences, Box 44, SE-230 53 Alnarp,
Sweden (S.M.)
In this work we have further characterized the first
mitochondrial nucleoside diphosphate kinase (mtNDPK) isolated from
plants. The mitochondrial isoform was found to be especially abundant in reproductive and young tissues. Expression of the pea (Pisum sativum L. cv Oregon sugarpod) mtNDPK was not affected by
different stress conditions. However, the pea mtNDPK was found to
interact with a novel 86-kD protein, which is de novo synthesized in
pea leaves upon exposure to heat. Thus, we have evidence for the
involvement of mtNDPK in mitochondrial heat response in pea in vivo.
Studies on oligomerization revealed that mtNDPK was found in complexes of various sizes, corresponding to the sizes of e.g. hexamers, tetramers, and dimers, indicating flexibility in oligomerization. This
flexibility, also found for other NDPK isoforms, has been correlated
with the ability of this enzyme to interact with other proteins. We
believe that the mtNDPK is involved in heat stress response in pea,
possibly as a modulator of the 86-kD protein.
1
This work was supported by the Carl Tryggers
Foundation and the Magnus Bergvall Foundation, Sweden.
2
Present address: Department of Plant Biology, Uppsala
Genetic Center, Swedish University of Agricultural Sciences, Box 7080, SE-750 07 Uppsala, Sweden.
*
Corresponding author; e-mail carina.knorpp{at}vbiol.slu.se; fax
46-18-673-273. .
© 2001 American Society of Plant Physiologists
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