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Plant Physiol, June 2001, Vol. 126, pp. 536-548
Flavonoid Accumulation Patterns of Transparent Testa Mutants of
Arabidopsis1
Wendy Ann
Peer,*
Dana E.
Brown,
Brian W.
Tague,
Gloria K.
Muday,
Lincoln
Taiz, and
Angus S.
Murphy
Department of Horticulture and Landscape Architecture, Purdue
University, West Lafayette, Indiana 47907 (W.A.P., A.S.M.); Department
of Biology, Wake Forest University, Winston-Salem, North Carolina 27109 (D.E.B., B.W.T., G.K.M.); and Biology Department, University of
California, Santa Cruz, California 95064 (L.T.)
Flavonoids have been implicated in the regulation of auxin
movements in Arabidopsis. To understand when and where flavonoids may
be acting to control auxin movement, the flavonoid accumulation pattern
was examined in young seedlings and mature tissues of wild-type
Arabidopsis. Using a variety of biochemical and visualization techniques, flavonoid accumulation in mature plants was localized in
cauline leaves, pollen, stigmata, and floral primordia, and in the
stems of young, actively growing inflorescences. In young Landsberg
erecta seedlings, aglycone flavonols accumulated
developmentally in three regions, the cotyledonary node, the
hypocotyl-root transition zone, and the root tip. Aglycone flavonols
accumulated at the hypocotyl-root transition zone in a developmental
and tissue-specific manner with kaempferol in the epidermis and
quercetin in the cortex. Quercetin localized subcellularly in the
nuclear region, plasma membrane, and endomembrane system, whereas
kaempferol localized in the nuclear region and plasma membrane. The
flavonoid accumulation pattern was also examined in transparent testa
mutants blocked at different steps in the flavonoid biosynthesis
pathway. The transparent testa mutants were shown to have precursor
accumulation patterns similar to those of end product flavonoids in
wild-type Landsberg erecta, suggesting that synthesis
and end product accumulation occur in the same cells.
1
This work was supported by the U.S. Department
of Agriculture (grant no. 94-37100-0755 to L.T.), by Sigma Xi (grant
to D.E.B.), and by the National Aeronautical and Space Administration
(grant no. NAG2 1203 to G.K.M.). The Wake Forest University Research and Publications Fund and Purdue Agricultural Research Programs (manuscript ID no. 16464) supported the publication costs.
*
Corresponding author; e-mail peer{at}hort.purdue.edu; fax
765-494-0391.
© 2001 American Society of Plant Physiologists
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