Plant Physiol, June 2001, Vol. 126, pp. 587-600

shygrl1 Is a Mutant Affected in Multiple Aspects of Photomorphogenesis¹

Department of Molecular, Cell, and Developmental Biology, P.O. Box 951606, University of California, Los Angeles, California 90095-1606 (M.S.-O., R.M.G., S.T., E.M.T.); and Department of Biological Sciences, California State University, 1250 Bellflower Boulevard, Long Beach, California 90640-3702 (J.A.B.)

We have used a counter-selection strategy based on aberrant phytochrome regulation of an Lhcb gene to isolate an Arabidopsis mutant designated shygrl1 (shg1). shg1 seedlings have reduced phytochrome-mediated induction of the Lhcb gene family, but normal phytochrome-mediated induction of several other genes, including the rbcS1a gene. Additional phenotypes observed in shg1 plants include reduced chlorophyll in leaves and additional photomorphogenic abnormalities when the seedlings are grown on medium containing sucrose. Mutations in the TATA-proximal region of the Lhcb1*3 promoter that are known to be important for phytochrome regulation affected reporter gene expression in a manner similar to the shg1 mutation. Our results are consistent with the possibility that the mutation either leads to defective chloroplast development or to aberrant phytochrome regulation. They also add to the evidence of complex interactions between light- and sucrose-regulated pathways.